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褐飞虱Nilaparvata lugens(S"非汉字符号"l)肌动蛋白基因3′-RACE及基因表达的RT-PCR检测

DOI: 10.7685/j.issn.1000-2030.2003.03.012, PP. 49-51

Keywords: 褐飞虱,肌动蛋白,3′-RACE,基因,表达

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Abstract:

通过锚定的3′RACE筛选实验,确定锚定效率最好的下游引物,用于褐飞虱各发育期肌动蛋白基因表达的RTPCR检测。结果表明3个锚定引物中,04227(5′>TCACACAGGAAACAGCTATGACTTTTTTTTTTTTTTA<3′)的锚定效率最好,可以扩增出5条褐飞虱的肌动蛋白基因3′末端片段,依其大小命名为BPHA、BPHB、BPHC、BPHD、BPHE。RTPCR检测表明BPHA从3龄开始到成虫期都有常量表达;BPHB、BPHC、BPHE从2龄开始到成虫期都有表达;BPHD在整个幼虫期都有表达,而在成虫期则检测不到

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