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二花脸猪和大白猪背最长肌中肌肉生长抑制素和生肌调节因子基因的表达及其性别特点

DOI: 10.7685/j.issn.1000-2030.2006.03.013, PP. 64-68

Keywords: 大白猪,二花脸猪,背最长肌,肌肉生长抑制素基因,生肌调节因子基因

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Abstract:

用相对定量RT-PCR方法研究大白猪和二花脸猪背最长肌中肌肉生长抑制素(Myostatin)和生肌调节因子(Myo-genin)基因表达的发育性变化并进行品种及性别间比较。研究结果表明大白猪公猪MyostatinmRNA表达水平在20日龄时达到高峰且显著高于相同日龄二花脸公猪(P<0.05),而二花脸公猪在45日龄时才显著升高,其他日龄品种间差异不显著。二花脸公猪和母猪出生后MyostatinmRNA表达水平均表现为上升,45日龄时达到高峰,120日龄时公母猪间差异显著(P<0.05)。二花脸公猪与大白猪公猪MyogeninmRNA的表达发育模式基本相同,均在20日龄达到最高值,品种间差异不显著。90日龄二花脸母猪MyogeninmRNA表达水平较公猪显著下调(P<0.05)。结果提示,猪背最长肌中Myostatin和Myogenin基因表达水平在猪出生后早期均较高,但没有出现随着日龄的增加而一直升高或降低的趋势;两种基因的表达在20日龄出现品种间差异;性成熟前后二花脸公母猪间表达有差异。

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