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三种茎线虫rDNA区的PCR-RFLP分析

DOI: 10.7685/j.issn.1000-2030.2006.03.008, PP. 39-43

Keywords: 甘薯茎线虫,鳞球茎茎线虫,食菌茎线虫,ITS-RFLP,酶切图谱

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Abstract:

采用PCR-RFLP技术对来自国内外的10个茎线虫属群体进行了限制性片段长度多态性分析(RFLP),发现甘薯茎线虫(Ditylenchusdestructor)不同群体间存在基因分化现象。来自山东的2个群体Dell和Dely的酶切图谱与来自其他地区的线虫群体相比明显不同。对甘薯茎线虫的近似种鳞球茎茎线虫(D.dipsaci)以及食菌茎线虫(D.myceliophagus)的分析结果显示,通过ITS-RFLP技术建立的酶切图谱可以很好地将3种茎线虫区分开来。

References

[1]  Thorne G.Ditylenchus destructor n.sp.,the potato rot nematode,and Ditylenchus dipsaci (Kuhn,1857) Filipjev,1936,the teasel nematode (Nematode:Tylenchidae)[J].Proc Helm in Soc.1945,12(2):27-33
[2]  Ferris V R,Ferris M,Faghihi J.Variation in spcer ribosomal DNA in some cyst-forming species of plant-parasitic nematodes[J].Fundamental and Applied Nematology.1993,16:177-184
[3]  尹光德,张云美.甘薯茎线虫病病原线虫的订正[J].山东大学学报(理学版).1983,12(4):117-127
[4]  Webster J M,Anderson R V,Baillie D L.DNA probes for differentiating isolates of the pinewood nematode species complex[J].Revue de Nematologie.1990,13(3):255-263
[5]  Beckenbach K,Smith M J,Webster J M.Tax anomic affinities and intra-and interspecific variation in Bursaphelenchus spp.as determined by polymerase chain reaction[J].Journal of Nematology.1992,24(1):140-147
[6]  林茂松.室内人工接种测定甘薯品种对马铃薯腐烂线虫的抗性[J].南京农业大学学报.1989,12(3):44-47
[7]  Wang J C,Yu B Y,Lin M S.Description of a new species (Nematoda,aphelenchoididae) isolated from wood packaging material[J].Acta Zootaxonomica Sinica.2005,30(2):314-319
[8]  Wendt K R,Vrain T C,Webster J M.Separation of three species of Ditylenchus and some host races of D.dipsaci by restriction fragment length polymorphism[J].Journal of Nematology.1993,25(4):555-563
[9]  丁再福,林茂松.甘薯、马铃薯和薄荷上的茎线虫的鉴定[J].植物保护学报.1982,9(3):169-172
[10]  Braasch H,Burger M W,Pastrik K H.Differention of three Bursaphelenchus species by means of RAPD-PCR[J].Nachrichtenblatt des Deutschen Pflanzenschutzdienstes.1995,47(12):310-314
[11]  Iwahori H,Tsuda K,Kanzaki N.PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease[J].Fundamental and Applied Nematology.1998,21(6):655-666
[12]  Powers T O,Todd T C,Burnell A M.The rDNA internal transcribed spacer region as a taxonomic maker for nematodes[J].Nematol.1997,29:441-450
[13]  林茂松,贺丽敏,文玲.甘薯形态结构对茎线虫病的抗性机制[J].中国农业科学.1996,29(3):8-12
[14]  Vrain T C,Warkarchuk D A,Lévesque A C.Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group[J].Fundamental and Applied Nematology.1992,15:563-573

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