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山核桃天然群体遗传结构的RAPD分析

DOI: 10.7685/j.issn.1000-2030.2006.03.003, PP. 12-17

Keywords: 山核桃,居群,遗传结构,RAPD

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Abstract:

利用随机扩增多态性DNA(RAPD)分子标记技术,分析了山核桃5个天然居群150个个体的遗传多样性和遗传结构,20条10bp的随机引物共扩增出252个位点,其中,多态性位点168个,多态位点百分率(PPB)为66.7%。居群水平Shannon′s多态性信息指数(I)在0.1992~0.2800间变化,物种水平I为0.4102;居群水平Ne′is基因多样性指数(H)介于0.1297~0.2051之间,物种水平H为0.2671。遗传变异计算显示,山核桃居群间基因分化系数(Gst)为0.3845。分子方差分析(AMOVA)表明,居群间基因分化系数为0.3413,大部分变异存在于居群内。居群间基因流(Nm)为0.9671,说明居群间基因交流相对较少。这一结果符合山核桃风媒、异交的繁育系统特点,但其居群间基因分化系数比异交植物的平均水平高。提示地理隔离、居群内近交及居群间有限的基因流可能是形成目前山核桃天然群体遗传结构的主要因素。

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