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水稻白叶枯病菌gacA基因的克隆、测序及敲除研究

DOI: 10.7685/j.issn.1000-2030.2006.03.007, PP. 33-38

Keywords: 水稻白叶枯病菌,gacA基因,基因敲除

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Abstract:

根据黄单胞菌gacA基因的同源性设计简并引物,采用PCR方法从水稻白叶枯病菌(Xanthomonasoryzaepv.oryzae,Xoo)中克隆了gacA同源基因,命名为gacAXoo。序列比较显示,该基因在黄单胞菌中是相对保守的。蛋白质保守结构域搜索表明,GacAXoo属于LuxR家庭的一员,具有与其他GacA蛋白相似的结构。通过同源重组的方法,构建了gacAXoo的插入突变体,为下一步研究gacAXoo的功能奠定了良好的基础。

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