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进境美国大豆幼苗中菜豆荚斑驳病毒的检测与鉴定

DOI: 10.7685/j.issn.1000-2030.2007.02.029, PP. 139-141

Keywords: 菜豆荚斑驳病毒,大豆幼苗,双抗体夹心ELISA,RT-PCR,序列测定

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Abstract:

从进口美国大豆中挑选具有典型斑驳症状的种子,在隔离温室内种植。出苗后,采用双抗体夹心酶联免疫吸附法(DAS-ELISA)进行菜豆荚斑驳病毒(beanpodmottlevirus,BPMV)的血清学检测。对BPMV血清学反应阳性的幼苗进一步采用反转录(RT)PCR方法和测序方法进行鉴定。结果从大豆幼苗中筛选并鉴定出菜豆荚斑驳病毒。

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