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转基因Alzheimer病模型小鼠组织中过渡元素的分布研究

DOI: 10.7685/j.issn.1000-2030.2007.02.024, PP. 116-121

Keywords: 淀粉样蛋白,过渡金属,Alzheimer病,转基因动物,自由基

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Abstract:

检测了转基因Alzheimer病(AD)模型小鼠脑、心、肝、脾、肺、肾、睾丸组织中Cu、Fe、Zn的含量。结果表明除肾脏外,AD小鼠各组织中Fe的含量都比对照组的高;骨和脑中的Cu比对照组多,其他组织中的Cu比对照组少;脾、肺中的Zn比对照多,其余组织的Zn均少于对照组。AD对过渡元素分布的影响有性别差异雄性个体中过渡元素含量异常的组织数目比雌性的多,与正常值偏离的程度也比雌性的严重。在脑切片中,AD个体Cu、Zn、Fe的平均含量均高于对照组。对于Cu与Zn,AD组除在顶皮层PtA、梨形-杏仁核皮层区低于对照组外,在皮层其他区域以及丘脑、下丘、海马中均高于对照;AD小鼠脑皮层、海马和下丘脑中的Fe多于对照。试验还发现,脑切片中Cu与Zn的分布地形图有相似性,Fe的分布与Cu、Zn的不同,揭示Cu、Zn有部分共同的运储载体与途径,而Fe的储运途径与之独立。AD诱发的淀粉样蛋白沉聚、血脑屏障通透增大及金属运储载体性能的改变对金属的异常分布起作用。

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