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黄瓜叶片生长期间内肽酶活性和同工酶变化及其生化特性

DOI: 10.7685/j.issn.1000-2030.2007.02.010, PP. 49-54

Keywords: 黄瓜,内肽酶,叶片衰老

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Abstract:

研究了黄瓜自然生长期间叶片可溶性蛋白质降解、内肽酶活性及其同工酶的变化,并对叶片衰老后期粗提液内肽酶的基本生化特性进行了研究。结果表明,黄瓜叶片内肽酶活性在叶片伸出5d时较低,叶片衰老后期(叶片伸出35d)显著升高,Rubisco蛋白发生明显降解;叶片全展以后叶片蛋白质含量与内肽酶活性显著负相关。黄瓜叶片粗提液总内肽酶的最适pH为7.0,最适温度为40℃,内肽酶活性可被Ca2+、Zn2+、半胱氨酸等激活。内肽酶抑制剂试验显示,丝氨酸类型的内肽酶活性占内肽酶总活性的57%左右。采用以明胶为底物的内肽酶梯度凝胶电泳活性染色方法,检测到叶片中至少有4种内肽酶同工酶,各同工酶在叶片生长过程中出现的时间和活性大小不同,丝氨酸型内肽酶同工酶活性随叶片生长明显增强,暗示该类内肽酶在黄瓜叶片衰老过程中可能具有重要作用。

References

[1]  Domínguez F,Cejudo F J.Pattern of endoproteolysis following wheat grain germination[J].Physiologia Plantarum.1995,95:253-259
[2]  Cruz de Carvalho M H,d’Arcy-Lameta A,Roy-Macauley H.Aspartic protease in leaves of common bean(Phaseolus vulgaris L.)and cowpea(Vigna unguiculata L.Walp):enzymatic activity,gene expression and relation to drought susceptibility[J].FEBS Letters.2001,492:242-246
[3]  Bushnell T P,Bushnell D,Jagendorf A T.A purified zinc protease of pea chloroplasts,EP1,degrades the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase[J].Plant Physiology.1993,103:585-591
[4]  Buchanan-Wollaston V,Ainsworth C.Leaf senescence in Brassica napus:cloning of senescence related genes by subtractive hybridisation[J].Plant Molecular Biology.1997,33:821-834
[5]  Rui Q,Xu L L.Degradation of rubulose-1,5-bisphosphate carboxylase/oxygenase in wheat leaves during dark-induced senescence[J].ACTA BOTANICA SINICA.2004,46(2):137-141
[6]  高玲,叶茂炳,张荣?.小麦旗叶老化期间的内肽酶[J].植物生理学报.1998,24(2):183-188
[7]  Distefano S,Palma J M,Gómez M.Characterization of endoproteases from plant peroxisomes[J].BIOCHEMICAL JOURNAL.1997,327:399-405
[8]  艾希珍,马兴庄,于立明.日光温室黄瓜叶片展开过程中光合特性的变化[J].中国农业科学.2005,38(3):558-564
[9]  Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry.1976,72:248-254
[10]  Makino A,Mae T,Ohira K.Relation between nitrogen and ribulose-1,5-bisphosphate carboxylase in rice leaves from emergence through senescence[J].Plant and Cell Physiology.1984,25(3):429-437
[11]  Laemmli U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J].NATURE.1970,227:680-685
[12]  Ant?o C M,Malcata F X.Plant serine proteases:biochemical,physiological and molecular features[J].Plant Physiology and Biochemistry.2005,43:637-650
[13]  Hou W C,Huang D J,Lin Y H.An aspartic type protease degrades trypsin inhibitors,the major root storage proteins of sweet potato(Ipomoea batatas(L.)Lam cv.Tainong 57)[J].Bot Bull Acad Sin.2002,43:271-276
[14]  Yamauchi Y,Sugimotob T,Sueyoshib K.A serine endopeptidase from cucumber leaves is inhibited by L-arginine,guanidino compounds and divalent cations[J].PHYTOCHEMISTRY.2001,58:677-682
[15]  Besse I,Wong J H,Kobrehel K.Thiocalsin:a thioredoxin-linked,substrate-specific protease dependent on calcium[J].Proceedings of the National Academy of Sciences(USA).1996,93:3169-3175
[16]  Liu X Q,Jagendorf A T.Neutral peptidases in the stroma of pea chloroplasts[J].Plant Physiology.1986,81:603-608
[17]  McConkey D J,Nicotera P,Hartzell P.Glucocorticoids activate a suicide process in thymocytes through an elevation of cytosolic Ca2+ concentration[J].Archives of Biochemistry and Biophysics.1989,269:365-370
[18]  Beers E P,Freeman T B.Proteinase activity during tracheary element differentiation in zinnia mesophyll cultures[J].Plant Physiology.1997,113:873-880
[19]  Jiang W B,Lers A,Lomaniec E.Senescence-related serine protease in parsley[J].PHYTOCHEMISTRY.1999,50:377-382
[20]  Rui Q,Xu L L.Characterization of endopeptidases in wheat leaves during dark-induced senescence[J].ACTA BOTANICA SINICA.2003,45(9):1049-1054

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