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不结球白菜BrCBF基因cDNA全序列克隆及结构特征分析

DOI: 10.7685/j.issn.1000-2030.2007.02.004, PP. 18-22

Keywords: 不结球白菜,CBF基因,克隆

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Abstract:

运用RT-PCR和RACE技术从不结球白菜中克隆到1个抗寒相关基因,命名为BrCBF,基因登陆号为DQ402470。其cDNA全长1003bp,含有648bp的完整开放阅读框,编码216个氨基酸,该基因编码的蛋白序列与拟南芥、荠菜、甘蓝型油菜编码的氨基酸序列有极高的同源性,且具有CBF典型的保守结构域AP2。

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