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H-FABP和HSL基因多态性对苏淮猪胴体性状的影响

DOI: 10.7685/j.issn.1000-2030.2008.03.021, PP. 107-112

Keywords: H-FABP基因,HSL基因,单倍型,苏淮猪,胴体性状

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Abstract:

应用PCR-RFLP方法检测了H-FABP、HSL基因在83头苏淮猪中的多态性分布。结果表明H-FABP基因的3个变异位点和HSL基因都存在多态性。H-FABPHinfⅠ位点HH基因型个体的背膘厚为19.81mm,极显著低于hh基因型个体(20.16mm)(P<0.01),Hh基因型个体的背膘厚为19.86mm,显著低于hh基因型个体(P<0.05)。HH基因型个体的瘦肉率为59.69%,极显著高于hh型个体(54.84%)(P<0.01),Hh基因型个体的瘦肉率为58.69%,显著高于hh型个体(P<0.05)。HH-Dd-Aa单倍型背膘最薄(19.73mm)、瘦肉率最高(60.92%),但没有达到显著水平。HSL基因GG基因型个体的背膘厚为19.79mm,极显著低于AA基因型个体(20.23mm)(P<0.01),AG基因型个体的背膘厚为19.88mm,显著低于AA基因型个体(P<0.05)。GG基因型个体的瘦肉率为59.89%,极显著高于AA型个体(54.04%)(P<0.01),AG基因型个体的瘦肉率为58.52%,显著高于AA型个体(P<0.05)。提示H-FABP、HSL基因可以作为胴体性状的候选基因用于猪的辅助选育。

 References

[1]  Knoll A,Stratil A,Nebola M.Chrachaterization of a polymorphism in exon Ⅰ of the porcine hormone sensitive lipase(LIPE)gene[J].Animal Genetics.1998,29:460-477
[2]  金访中,黄瑞华,王松均.长白猪胴体组成与瘦肉率的关系[J].南京农业大学学报.2000,23(1):55-57
[3]  庞卫军,孙世铎,李影.西部地区主要猪种和野猪H-FABP基因分子标记与IMF含量关系[J].遗传.2005,27(3):351-356
[4]  Urban T,Mikolasova R,Kuciel J.A study of associations of the H-FABP genotypes with fat and meat production of pigs[J].Journal of Applied Genetics.2002,43(4):505-509
[5]  李长龙,潘玉春,孟和.H-FABP、MC4R、ADD1基因多态性在3个猪群中分布及其与肌内脂肪和背膘的相关研究[J].遗传.2006,28(2):159-164
[6]  雷明刚,吴珍芳,邓昌彦.不同品种猪HSL基因5′-UTR 和外显子Ⅰ片段的克隆测序及多态性分析[J].遗传学报.2005,32(4):354-359
[7]  Schaap F G,van der Vusse G J,Glatz J F C.Fatty acid-binding proteins in the heart[J].Molecular and Cellular Biochemistry.1998,180(1-2):43-51
[8]  Gerbens F,van Erp A J M,Harders F L.Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs[J].Journal of Animal Science.1999,77:846-852
[9]  Frayn K N,Coppack S W,Fielding B A.Coordinated regulation of hormone-sensitive lipase and lipoprotein lipase in human adipose tissue in vivo:implications for the control of fat storage and fat mobilization[J].Advances in Enzyme Regulation.1995,35:163-178
[10]  Mellink C H,Lahbib-Mansais Y,Yerle M.Localization of four new markers to pig chromosomes 1,6 and 14 by radioactive in situ hybridization[J].Cytogenetics and Cell Genetics.1993,64:256-260
[11]  Egan J J,Greenberg A S,Chang M K.Mechanism of hormones-stimulated lipolysis in adipocytes:translocation of hormone-sensitive lipase to lipid storage droplet[J].Proceedings of the National Academy of Sciences(USA).1992,89:8537-8541
[12]  陈润生.优质猪肉的指标及其度量方法[J].养猪业.2002(3):1-5
[13]  林万华,黄路生,任军.中外十个猪种H-FABP基因遗传变异的研究[J].遗传学报.2002,29(1):12-15
[14]  Li Jia-qi,Zhang Hao,Wang Chong.Genetic effects of H-FABP gene on some pig economic important traits in a F2 resource population[J].Agricultural Sciences In China.2003,2(3):321-324
[15]  吴珍芳,熊远,Harbitz I.猪HSL基因多态性研究及其部分DNA片段的测序[J].遗传学报.2000,27(8):686-690
[16]  杨阳,姜运良,胡晓湘.猪激素敏感脂肪酶基因(HSL)外显子Ⅷ的SSCP分析[J].农业生物技术学报.2001,9(2):142-144
[17]  Gerbens F,Rettenberger G,Lenstra J A.Characterization,chromosomal localization,and genetic variation of the porcine heart fatty acid-binding protein gene[J].Mammalian Genome.1997,8(5):328-332
[18]  Harbitz I,Chowdhary B,Thomsen P D.Assignment of the porcine calcium release channel gene,a candidate for the malignant hyperthermial locus,to the 6p11-q21 segment of chromosome 6[J].Genomics.1990,8(2):243-248
[19]  Holst L S,Langin D,Mulder H.Molecular cloning,genomic organization,and expression of a testicular isoform of hormone-sensitive lipase[J].Genomics.1996,35(3):441-447

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