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栗疫病菌单个营养体不亲和性基因差异对弱毒性病毒传递的影响

DOI: 10.7685/j.issn.1000-2030.2009.02.014, PP. 65-69

Keywords: 栗疫病菌,营养体不亲和性,vic基因,传递,弱毒性病毒

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Abstract:

研究了栗疫病菌(Cryphonectriaparasitica)菌株间单个营养体不亲和性(vegetativeincompatibility,vic)基因差异对弱毒性病毒传递的影响。将含病毒菌株与具单个vic基因座位差异的野生型菌株配对培养,10d后调查菌株间的病毒传递率。结果表明,相同vic基因型菌株间的病毒传递率为100%;在1个vic座位上的基因不同通常会降低病毒的传递率,在vic-Cc座位上基因差异菌株间的病毒平均传递率为85.93%,而在vic-Dd座位上基因不同菌株间病毒的平均传递率仅为10.30%,在vic-Aa、vic-Bb座位上基因不同的菌株间病毒平均传递率分别为44.12%、47.93%。vic-Bb座位上基因不同菌株间的病毒正反向传递率相差非常大,由vic-b菌株向vic-B菌株的平均病毒传递率为83.13%,而从vic-B菌株向vic-b菌株的平均病毒传递率只有23.15%;其他3个vic座位上基因不同菌株间的病毒传递率也具有方向性,但不明显。

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