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不结球白菜雌蕊蛋白质双向电泳技术体系的建立

DOI: 10.7685/j.issn.1000-2030.2012.01.003, PP. 14-20

Keywords: 不结球白菜,雌蕊,蛋白质,双向电泳

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Abstract:

以不结球白菜品种’矮脚黄’的雌蕊为材料,从蛋白质提取、IEF程序、IPG胶条pH范围和染色方法等方面进行比较,利用Tris-HCl提取方法,使用pH4~7IPG胶条和改进的等电聚焦程序,改良银染方法,建立了适用于不结球白菜雌蕊蛋白质的双向电泳技术体系,并使用该体系对开花前2d的花蕾雌蕊和开花2d后的雌蕊蛋白质进行比较分析,获得187个差异蛋白点。

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