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HSP70基因多态性对矮小型黄羽肉鸡热耐受性的影响

DOI: 10.7685/j.issn.1000-2030.2013.06.013, PP. 80-88

Keywords: ,HSP70基因,单核甘酸多态性,生理指标,免疫指标

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Abstract:

采用PCR产物直接测序和PCR-RFLP检测的方法,选取HSP70基因的C.-69A>G、C.+258A>G、C.+276C>G、C.+909C>A、C.+1044A>G、C.+1437C>T和C.+1476C>T7个多态位点,分别在15和35℃条件下对矮小型黄羽肉鸡纯系母鸡进行与热耐受性相关的部分生理与免疫指标的关联分析。结果表明热应激状态下(35℃),C.+276C>G位点与CD3+T细胞含量显著相关(PT位点与CD4+T细胞含量极显著相关(PT位点的CT基因型个体H/L值显著低于CC基因型个体(PG位点的GG基因型个体CD4+T细胞含量显著高于AG基因型个体(PG位点与CD4+T细胞含量显著相关(PT位点与CD3+T细胞含量、皮质酮值显著相关(PG、C.+276C>G、C.+1044A>G、C.+1437C>T和C.+1476C>T5个位点与部分生理、免疫指标显著相关。其中C.+1437C>T位点与多个生理、免疫指标都有显著或极显著相关,表明该位点对矮小型黄羽肉鸡的热耐受性具有潜在的遗传效应。

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