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葡萄谱系特有基因生物信息学识别、基因结构与表达特性分析

DOI: 10.7685/j.issn.1000-2030.2013.06.004, PP. 19-24

Keywords: 葡萄,谱系特有基因,生物信息学,基因表达

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Abstract:

以葡萄全基因组29971蛋白序列为查询序列,与包括葡萄在内的31个已测序植物蛋白序列、TIGR和NCBI数据库中的EST和非冗余蛋白质序列进行非同源性比对分析,保留E值大于0.01的序列,去除重复序列、转座子和注释不准确的基因序列,保留的非同源基因识别为葡萄谱系特有基因;并对谱系特有基因的结构和具有芯片表达信息的谱系特有基因的表达特性进行了分析。结果表明共识别了2009个葡萄谱系特有基因,与葡萄保守基因相比较,葡萄谱系特有基因平均外显子数量较少,为2个;平均外显子长度较短,为232bp;平均内含子长度较长,为1723bp,与拟南芥、水稻等谱系特有基因结构特点相似,谱系特有基因可能具有相似的起源和进化模式。在47个具有芯片表达信息和相应UniGene信息的基因中,Vv04s0023g01710、Vv00s0187g00160、Vv17s0000g09800、Vv14s0083g00260分别在叶片、茎、细胞培养物、果实中相对表达量最高,相对表达量分别为2120、2282、5566、1428;有25个谱系特有基因仅在某一器官中表达,表明谱系特有基因具有器官特异性特点;分析34个谱系特有基因在4种非生物胁迫下基因表达情况,表明大部分基因表达受到明显抑制或不受诱导,推测非生物胁迫影响了这些谱系特有基因正常功能,也说明这些谱系特有基因较少参与非生物胁迫过程。

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