全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

红树甜菜碱/脯氨酸转运蛋白基因Bet/ProT2转化水稻及耐盐性的研究

DOI: 10.7685/j.issn.1000-2030.2013.05.009, PP. 51-58

Keywords: 水稻,红树,甜菜碱/脯氨酸转运蛋白,耐盐性,亚细胞定位

Full-Text   Cite this paper   Add to My Lib

Abstract:

克隆了红树植物’白骨壤’中甜菜碱/脯氨酸转运蛋白基因(Bet/ProT2),采用根瘤农杆菌介导的方法,将携带有GFP报告基因的35S-Bet/ProT2-GFP融合质粒转化洋葱表皮细胞,亚细胞定位分析表明,Bet/ProT2是定位在细胞质膜上的跨膜蛋白。进一步利用农杆菌介导法将Bet/ProT2转入粳稻‘日本晴’,鉴定结果表明,Bet/ProT基因已经整合到‘日本晴’的基因组中并有效表达。转Bet/ProT基因水稻的耐盐性明显提高,在甜菜碱和脯氨酸同时或分别存在的条件下,转基因水稻可以在含有150mmol?L-1NaCl的液体培养基中正常生长,同时叶片中H2O2含量较低;而野生型水稻在相同处理条件下出现明显枯萎,叶片中H2O2大量积累。结论红树Bet/ProT2基因能通过吸收甜菜碱和/或脯氨酸来降低氧化伤害,显著提高转基因水稻的耐盐能力。

References

[1]  Rhoades J D,Loveday J.Salinity in Irrigated Agriculture[M]//Steward B A,Nielsen D R.Irrigation of Agricultural Crops.American Society of Agronomists,USA,1990,30:1089-1142
[2]  Liu W H,Fairbairn D J,Reid R J,et al.Characterization of two HKT1 homologues from Eucalyptus camaldulensis that display intrinsic osmosensing capability[J].Plant Physiol,2001,127:283-294
[3]  Zhang H X,Blumwald E.Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit[J].Nature Biotechnol,2001,19:765-768
[4]  Moxas V P.Over-expression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress[J].Nat Biotechnol,1997,15:988-991
[5]  Chen T H H,Murata N.Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes[J].Current Opinion in Plant Biology,2002,5(3):250-257
[6]  林鹏.中国红树林生态系[M].北京:科学出版社,1997:5-24,133-147
[7]  Waditee R,Hibino T,Tanaka Y,et al.Functional characterization of betaine/proline transporters in betaine-accumulating Mangrove[J].Biol Chem,2002,277(21):18373-18382
[8]  Yoshida S,Forno D A,Cock J.Laboratory Manual for Physiological Studies of Rice[M].Manila:Int Rice Res Inst,1972
[9]  Tarlow J K,Blakemore A I F,Lennard A,et al.Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86 bp tandem repeat[J].Hum Genet,1993,91(4):403-404
[10]  Orozco-Cardenas M L,Ryan C.Hydrogen peroxideid generated systemically in plant leaves by wounding and systemin via the octapeptide pathway[J].Proc Natl Acad Sci USA,1999,96:6553-6557
[11]  李亮,程彦伟,张炜,等.蓝色温和凝胶双向电泳用于分析嗜盐菌质膜蛋白复合物[J].生物化学与生物物理进展,2010,37(5):558-567
[12]  班青宇,耿建峰,侯喜林,等.不结球白菜叶片脯氨酸与可溶性蛋白含量的QTL分析[J].南京农业大学学报,2010,33(2):35-38.doi:10.7865/j.issn.1000-2030.2010.02.007
[13]  韩志平,郭世荣,高新昊,等.盐胁迫对西瓜幼苗生长、叶片光和色素和脯氨酸含量的影响[J].南京农业大学学报,2008,31(2):32-36.doi:10.7865/j.issn.1000-2030.2008.02.007
[14]  Chen T H H,Murata N.Glycine betaine:an effective protectant against abiotic stress in plants[J].Trends in Plant Science,2008,13(9):499-505
[15]  Hoque M A,Banu M N A,Nakamura Y,et al.Proline and glycine betaine enhance antioxidant defense and methylxanthine detoxification systems and reduce NaCl-induced damage in cultured tobacco cells[J].Journal of Plant Physiology,2008,165:813-824
[16]  Al-Taweel K,Iwaki T,Yabuta Y,et al.A bacterial transgene for catalase protects translation of D1 protein during exposure of salt-stressed tobacco leaves to strong light[J].Plant Physiol,2007,145:258-265
[17]  Papageorgiou G C,Murata N.The unusually strong stabilizing effects of glycine betaine on the structure and function of the oxygen-evolving photosystem Ⅱ complex[J].Photosynthesis Research,1995,44:243-252
[18]  Nuccio M L,Russell B L,Nolte K D,et al.The endogenous choline supply limits glycine betaine synthesis in transgenic tobacco expressing choline monooxygenase[J].The Plant Journal,1998,16:487-496
[19]  Chen T H H,Murata N.Enhancement of tolerance to abiotic stress by metabolic engineering of betaines and other compatible solutes[J].Current Opinion in Plant Biology,2002,5(3):250-257
[20]  Goel D,Singha A K,Yadava V,et al.Transformation of tomato with a bacterial codA gene enhances tolerance to salt and water stresses[J].Journal of Plant Physiology,2011,168:1286-1294
[21]  Chen T H H,Murata N.Glycine betaine protects plants against abiotic stress:mechanisms and biotechnological applications[J].Plant Cell and Environment,2011,34:1-20
[22]  王建飞,陈宏友,杨庆利,等.盐胁迫浓度和胁迫时的温度对水稻耐盐性的影响[J].中国水稻科学,2004,18(5):449-454
[23]  杨福,梁正伟,王志春.水稻耐盐碱鉴定标准评价及建议与展望[J].植物遗传资源学报,2011,12(4):625-628

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133