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与水稻互作的枯草芽孢杆菌OKB105菌株胞内全蛋白双向电泳条件的优化

DOI: 10.7685/j.issn.1000-2030.2013.05.011, PP. 65-70

Keywords: 枯草芽孢杆菌OKB105菌株,水稻,胞内全蛋白,双向电泳

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Abstract:

用枯草芽孢杆菌(Bacillussubtilis)OKB105菌株菌悬液处理水稻种子及幼苗,发现OKB105菌株能够显著促进水稻生长。为了研究枯草芽孢杆菌的促生机制,建立了水稻与OKB105菌株的互作体系,并对互作后的OKB105菌株胞内全蛋白的双向电泳条件进行了优化。结果表明分离胶浓度为10%,等电聚焦(IEF)程序中最高电压8000V,聚焦60000Vh且增加低电压慢速除盐步骤能够提高双向电泳图谱质量,为蛋白质组学水平上研究水稻与枯草芽孢杆菌的互作奠定了良好的基础。

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