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禾本科植物内生真菌研究17:黄山大雀麦-内生真菌 新共生体的生物学特征

DOI: 10.7685/j.issn.1000-2030.2013.05.010, PP. 59-64

Keywords: 大雀麦,内生真菌,形态学特征,系统发育学特征

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Abstract:

本研究在2011和2012年夏季从安徽省黄山市4个采样点采集大雀麦(BromusmagnusKeng)559株,镜检结果表明含菌率为48.0%~77.8%。含菌植物样品经表面消毒分离得到31株菌,根据形态学特征将这些菌株分为2个菌群快速生长(fastgrowing,FG)菌群,生长速度为每周16.0~18.8mm;慢速生长(slowgrowing,SG)菌群,生长速度为每周4.3~5.3mm。FG菌群分生孢子(4.5~5.1)μm×(2.9~3.6)μm及分生孢子梗(15.8~24.6μm)等无性形态结构比SG菌群略大。从同一地点、同种宿主植物中分离得到分属于FG和SG菌群的不同菌株,显示出一定的遗传多样性。在基于tubB基因片段构建的系统发育树中,FG和SG菌群菌株间未表现出差异,全部菌株在Epichlo?bromicola/E.yangzii(EBY)中独立形成后验概率值为0.95的一支,区别于其他种的菌株,表明菌株出现生长速度显著差异的时间可能并不久远,这些菌株与大雀麦有可能是一个新的epichloid真菌-宿主植物组合。

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