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南京农业大学学报 2013

‘砀山酥梨’叶片中Fer2基因的克隆与表达分析

DOI: 10.7685/j.issn.1000-2030.2013.05.006, PP. 33-38

钟晨,苏军,汤婷婷,丁伟,朱立武,贾兵

Keywords: 砀山酥梨,缺铁,Fer2基因,克隆,表达分析

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Abstract:

分别以轻度、中度和重度缺铁的‘砀山酥梨’叶片cDNA为模板,正常叶片为对照,运用RACE技术,克隆Fer2基因的cDNA全长为1052bp,编码区828bp,编码276个氨基酸。采用Protparam、InterProScan和ProtFun2.2Server等生物学信息软件分析蛋白质理化性质和结构域特征,结果表明Fer2与‘翠冠’梨叶片的Fer2蛋白和Fer4蛋白等具有较高的相似性,故命名该基因为PbFer2。推测蛋白质相对分子质量为3054.3,理论等电点为5.80。通过Real-timePCR技术,分析PbFer2基因表达量,结果表明轻度缺铁会提高PbFer2的表达量,随着缺铁程度的增加,PbFer2表达量显著下降。

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