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芹菜过敏原蛋白Api g 1基因的克隆与表达分析

DOI: 10.7685/j.issn.1000-2030.2013.02.003, PP. 13-19

Keywords: 芹菜,过敏原蛋白,Apig1基因,克隆,实时定量PCR,基因表达

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Abstract:

以2个芹菜(Apiumgraveolens)品种‘津南实芹’和‘美国西芹’为试验材料,分别克隆出过敏原蛋白Apig1基因。序列分析表明,‘津南实芹’和‘美国西芹’中的过敏原蛋白基因Apig1均含有1个480bp的开放阅读框及1个148bp的内含子,分别编码159个氨基酸,二者有2个核苷酸位点的差异,编码的氨基酸有1个位点差异。‘津南实芹’和‘美国西芹’的过敏原蛋白Apig1与胡萝卜、欧芹等植物的过敏原蛋白相似度较高,在保守区域有7个甘氨酸残基,空间结构上由3个螺旋和7个折叠组成。实时定量PCR表达分析显示,该基因在主根中表达较高,茎其次,其他部位表达较弱,具有明显的组织特异性。

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