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小菘菜游离小孢子培养和植株再生

DOI: 10.7685/j.issn.1000-2030.2013.02.002, PP. 7-12

Keywords: 小菘菜,游离小孢子培养,胚状体,植株再生

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Abstract:

以7个基因型的小菘菜品种为供试材料,采用游离小孢子培养技术,研究基因型、培养方法、激素及AgNO3对小孢子胚芽诱导率和植株再生的影响。结果表明基因型是影响小菘菜小孢子胚芽诱导率的最重要的因子;小菘菜小孢子培养初期用170g？L-1蔗糖处理1d后,更换培养基能显著提高小孢子的胚芽诱导率;0.05mg？L-16-BA和0.2mg？L-1NAA能促进小菘菜小孢子胚的发生;添加0.1mg？L-1GA3和0.3或0.4mg？L-1AgNO3可显著提高小菘菜小孢子胚的胚芽诱导率和平均每胚出芽数。

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