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鸡骨髓源树突状细胞体外诱导培养及鉴定

DOI: 10.7685/j.issn.1000-2030.2013.02.018, PP. 110-114

Keywords: 树突状细胞,体外培养,鉴定

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Abstract:

为建立鸡骨髓源树突状细胞(DC,dendriticcells)体外培养方法,用重组鸡粒细胞-巨噬细胞集落刺激因子(rGM-CSF)和重组鸡白细胞介素4(rIL-4)体外诱导鸡骨髓细胞分化为DC,然后通过形态观察、表型鉴定及功能分析来初步鉴定所培养的DC。试验结果表明培养7d后,光学显微镜下观察到细胞表面不规则,有显著的树突状突起,呈典型的DC形态,流式细胞仪测得细胞表面CD11c和MHCⅡ分子的表达量分别为69.3%和63.0%。经脂多糖或CpG-ODN刺激24h后的DC,其表面成熟分子标志CD40和CD86上调表达,同时其刺激同种异体T细胞增殖的能力显著增强(P<0.01)。结论本试验建立的方法能在体外制备出大量具有较高纯度的鸡骨髓源DC,并具有体内DC的生物学特征。

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