[目的] 研究外源生长调节剂处理对梨杂交后代的花芽分化、成花相关基因表达及营养物质积累的影响。[方法] 以5年生的‘满天红’ב砀山酥梨’杂交后代中尚未开花的实生个体为材料,在花芽分化期进行6-BA和乙烯利(Eth)喷施处理(100 mg？kg-1 6-BA,1 000 mg？kg-1 Eth,100 mg？kg-1 6-BA+1 000 mg？kg-1 Eth),以清水为对照。[结果] 不同生长调节剂处理均能促进杂交后代实生苗成花,成花数量显著增加,其中6-BA和乙烯利混合液处理形成的花芽个数最多。各处理均促进了花芽分化期间成花相关基因FLOWERING LOCUS T(FT)和LEAFY(LFY)的表达,抑制了TERMINAL FLOWER 1(TFL1)基因的表达;提高了花芽内玉米素(ZT)和ABA的含量,降低了GA3和IAA的含量,提高了花芽的淀粉含量和C/N比值。[结论] 综合分析表明,基因表达、内源激素和营养物质之间相互影响,共同调控杂交后代的成花。[Objectives] This study aims at exploring the effects of growth regulators on floral differentiation, expression of flowering related genes and nutrient accumulation of pear hybirds. [Methods] This study took ‘Mantianhong’× ‘Dangshansuli’ progenies as materials, and all the individuals were five years old but still have no flower. We sprayed the 6-BA and ethephon(Eth)(100 mg？kg-1 6-BA, 1 000 mg？kg-1 Eth, 100 mg？kg-1 6-BA+1 000 mg？kg-1 Eth)with water control during the period of flower bud differentiation. [Results] All different growth regulator treatments can facilitate the florescence of the progenies. The mixed 6-BA and ethephon treatment increased the most flower buds among three treatments. All treatments promoted the expression of FLOWERING LOCUS T(FT) and LEAFY(LFY) genes during floral bud differentiation, and inhibited the expression of TERMINAL FLOWER 1(TFL1) gene;the treatments enhanced the content of ZT and ABA, reduced the hormone content of GA3 and IAA, and improved the content of starch and the ratio of C/N in floral buds. [Conclusions] The synthesis analysis revealed that gene expression, endogenous hormone and nutriment influenced each other, and they regulated floral initiation of hybrid progenies together
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