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罗非鱼胰蛋白酶Ⅱ cDNA克隆与序列分析

DOI: 10.7685/j.issn.1000-2030.2005.01.032, PP. 139-142

Keywords: 罗非鱼,胰蛋白酶Ⅱ,cDNA,克隆

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Abstract:

应用3′RACE方法对尼罗罗非鱼(Oreochromisniloticus)和奥利亚罗非鱼(Oreochromisaureus)胰蛋白酶ⅡcDNA进行了克隆并进行序列测定和分析。结果表明,胰蛋白酶Ⅱ序列中均具有催化活性必需的高度保守的催化三联体氨基酸残基(His、Asp、Ser)和构成二硫键的10个半胱氨酸残基,以及决定底物特异性的保守性氨基酸残基―――天冬氨酸残基和S1结合袋。奥利亚罗非鱼和尼罗罗非鱼胰蛋白酶ⅡmRNA序列以及氨基酸序列同源性分别为964%和975%。

References

[1]  Rypniewski W,Perrakis A,Vorgias C E.Evolutionary divergence and conservation of trypsin[J].Protein Engineering.1994,7:57-64
[2]  Ahsan M N,Funabara D,Watabe S.Molecular cloning and characterization of two isoforms of trypsinogen from anchovy pyloric ceca[J].Marine Biotechnology.2001,3:80-90
[3]  Szabo E,Rocskei Z,Naray-Szabo G.The three-dimensional structure of Asp189Ser trypsin provides evidence for an inherent structural plasticity of the proteases[J].European Journal of Biochemistry.1999,263:20-26
[4]  Asgeirsson B,Fox J W,Bjarnason J B.Purification and characterization of trypsin from the poikilotherm Gadus morhua[J].European Journal of Biochemistry.1989,180:85-94
[5]  Hedstrom L,Lin T,Fast W.Hydrophobic interactions control zymogen activation in the trypsin family of serine proteases[J].Biochemistry.1996,35:4515-4523
[6]  Smalas A O,Heimstad E S,Hordvikn A.Cold adaption of enzymes:structural comparison between salmon and bovine trypsins[J].Proteins:Structure,Function,And Bioinformatics.1994,20:149
[7]  Gildberg A,Overbo K.Purification and characterization of pancreatic elastase from Atlantic cod (Gadus morhua)[J].Comparative Biochemistry and Physiology.1990,978:775-782

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