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热应激下肉鸡心肌的损伤和热休克蛋白的定位

DOI: 10.7685/j.issn.1000-2030.2005.01.030, PP. 131-134

Keywords: 热休克蛋白,心肌纤维,免疫组织化学,肉鸡

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Abstract:

将90只肉鸡随机分成A组(对照)、B组(热应激6h)、C组(热应激12h)、D组(热应激18h)、E组(热应激24h)和F组(热应激48h)。对各组受试鸡心肌进行病理组织学检查,并利用4种HSP单克隆抗体(HSP27、HSP60、HSC70和HSP86)对心肌纤维进行免疫组织化学染色。实验结果显示,热应激时心肌纤维的病理组织学损伤主要表现为心肌纤维变性和心肌纤维断裂;HSP27、HSP60、HSC70和HSP86在心肌纤维中的定位不尽相同,HSP27、HSC70和HSP86在心肌纤维中有强阳性表达,但HSP60的阳性染色较弱。提示HSPs在心肌纤维内的广泛分布对保护心肌纤维方面可能起重要的作用。

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