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半胱胺对羊毛生长及皮肤中GH受体、IGF-1和IGF-1型受体基因表达的影响

DOI: 10.7685/j.issn.1000-2030.2005.01.018, PP. 80-84

Keywords: 半胱胺,绵羊,羊毛,生长激素受体,胰岛素样生长因子1,胰岛素样生长因子1受体

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Abstract:

罗米丽×中国美利奴(新疆军垦型)杂交一代断奶母羔214只,随机分成两组,在日粮中分别添加35(试验组)和0g?kg-1(对照组)生长调节剂CT2000(半胱胺盐酸盐),实验期为120d。于试验前和试验结束时称重,采毛和皮肤样品,测定母羔生长速度,羊毛长度、细度、强度、弯曲度和羊毛油汗颜色,并用反转录多聚酶链式反应(RTPCR)方法,定量分析绵羊皮肤中生长激素受体(GHR)、胰岛素样生长因子1(IGF1)和胰岛素样生长因子1型受体(IGF1R)mRNA的相对丰度。实验结果显示,与对照组相比较,试验组平均日增重提高1277%(P005),羊毛油汗颜色和羊毛弯曲度的正常百分率显著提高(P<005);皮肤中GHRmRNA表达量增加11057%(P<001),IGF1、IGF1RmRNA表达量无明显变化。结果表明,半胱胺(CT2000)能够显著提高羔羊生长性能,促进羊毛生长,其机制可能与提高皮肤中GHR水平有关。

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