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Ca /CaM在壳聚糖调控不结球白菜离体叶片氨同化关键酶活性中的作用

DOI: 10.7685/j.issn.1000-2030.2005.01.008, PP. 34-38

Keywords: 壳聚糖,不结球白菜,谷氨酸脱氢酶,谷氨酰胺合成酶,钙信使系统

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Abstract:

用壳聚糖(CTS)、Ca2+、Ca2+螯合剂(EGTA)、异博定(Vp)、LaCl3、氯丙嗪(CPZ)、二硫苏糖醇(DTT)和腺苷三磷酸(ATP)单独或混合处理不结球白菜离体叶片。结果表明,CTS、Ca2+、DTT和ATP单独处理均能提高谷氨酸脱氢酶(GDH)和谷氨酰胺合成酶(GS)活性;而EGTA、异博定、LaCl3和CPZ单独处理则使GDH和GS活性降低。CTS可以与外源Ca2+及DTT和ATP互作促进GDH和GS活性;而在EGTA、质膜Ca2+通道阻断剂异博定和LaCl3以及CaM拮抗剂氯丙嗪分别存在下,则降低GDH和GS的活性。说明CTS在调控氨同化关键酶GDH和GS活性时,可能有Ca2+信号系统的参与,从而使得壳聚糖处理的不结球白菜的品质和单株鲜重有所提高。

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