全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

相关文章

更多...

马立克氏病病毒VP22基因siRNA筛选体系的构建

DOI: 10.7685/j.issn.1000-2030.2006.04.030, PP. 143-145

Keywords: 马立克氏病病毒,VP22基因,筛选体系,小干扰RNA

Full-Text   Cite this paper   Add to My Lib

Abstract:

将马立克氏病病毒(MDV)超强毒株VP22基因插入真核表达载体pEGFP-C1中EGFP基因的下游,构建EGFP-VP22融合基因真核表达载体pEGFP-VP22。将其转染至中国仓鼠卵巢细胞(CHO-k1)中,通过荧光显微镜观察EGFP的表达情况,并用RT-PCR检测VP22基因的表达。检测证实EGFP和VP22在CHO细胞中均得到了有效表达。

 References

[1]  Agami R.RNAi and related mechanisms and their potential use for therapy[J].Current Opinion in Chemical Biology.2002,6(6):829-834
[2]  Elbashir S M,Harborth J,Lendeckel W.Duplexes of 21 nucleotide RNAs mediate RNA interference in cultured mammalian cells[J].NATURE.2001,411:494-498
[3]  缪德年,王秀花,姚惠娟.UL49基因克隆与序列比较[J].上海农业学报.2005,21(2):24-27
[4]  Lauren A O,Jill A C,Kirk C.Marek’s disease virus VP22:subcellular localization and characterization of carboxyl terminal deletion mutations[J].VIROLOGY.2002,292:235-240
[5]  Dorange F,Mehdaoui S E,Pichon C.Marek’s disease virus(MDV) homologues of herpes simplex virus type 1 UL49 (VP22) and UL48 (VP16) genes:high-level expression and characterization of MDV-1 VP22 and VP16[J].Journal of General Virology.2000,81:2219-2230
[6]  Labas Y A,Gurskaya N G,Yanushevich Y G.Diversity and evolution of the green fluorescent protein family[J].Proceedings of the National Academy of Sciences of the United States of America.2002,99(7):4256-4261
[7]  缪德年,王秀花,封江南.马立克氏病病毒超强毒UL49基因siRNA表达质粒的构建与鉴定[J].南京农业大学学报.2005,28(3):137-139

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133