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伊莎笼养蛋鸡胰岛素样生长因子I限制性片断多肽性与骨矿的研究

DOI: 10.7685/j.issn.1000-2030.2006.04.023, PP. 111-114

Keywords: 伊莎笼养蛋鸡,胰岛素样生长因子-I,多态性,骨密度

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Abstract:

选择58周龄高产期伊莎笼养蛋鸡90羽,应用PCR扩增鸡IGF-I启动子上游7kb处基因片段,用PstI消化产生限制性片段长度多态性,分析IGF-I多态性与胫骨、股骨、肱骨骨密度之间的关系。结果表明,伊莎蛋鸡PP、Pp、pp基因型频率分别为0.256、0.311、0.433,P和p等位基因频率分别为0.4111和0.5889。方差分析显示,肱骨的骨密度与IGF-I基因多态性相关,PP基因型组的骨密度和骨矿含量均显著大于pp基因型(P<0.05),股骨、胫骨基因型间无显著差异。PP基因型体质量显著大于Pp基因型。表明伊莎笼养蛋鸡IGF-I基因多态性和肱骨骨密度显著相关,P等位基因具有一定的优良骨质指针作用。

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