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IGF-I和EGF对绵羊毛囊体外培养的影响

DOI: 10.7685/j.issn.1000-2030.2006.02.030, PP. 138-141

Keywords: 绵羊,毛囊,细胞生长因子

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Abstract:

在常规培养液中分别添加不同剂量的细胞生长因子IGFⅠ(100ng.mL-1、10ng.mL-1、1ng.mL-1、0.1ng.mL-1)和EGF(100ng.mL-1、20ng.mL-1、2ng.mL-1、0.2ng.mL-1),观察毛囊体外培养过程中生长速度及毛囊形态结构的变化。结果表明,10ng.mL-1IGFⅠ和20ng.mL-1EGF对毛囊均具有明显的促生长作用,10ng.mL-1IGFⅠ有刺激毛囊生长正向调节作用,20ng.mL-1EGF可促进毛囊外根鞘细胞分化,并诱导毛囊由生长期提前进入退行期。10ng.mL-1IGFⅠ和20ng.mL-1EGF混合对毛囊生长有明显的促进作用。

References

[1]  Harris P M,McBride B W,Gurnsey M P.Direct infusion of a variant of insulin-like growth factor-Ⅰ into skin of sheep and effects on local blood flow,amino acid utilisation and cell replication[J].Endocrinology.1993,139:463-472
[2]  Stewart C E H,Rotwein P.Growth,differentiation,and survival:multiple physiological functions for insulin-like growth factors[J].Physiological Reviews.1996,76:1005-1026
[3]  Stenn K S,Lawrence L,Veis D.Expression of the bcl-2 protooncogene in the cycling adult mouse hair follicle[J].International Journal of Dermatology.1994,103:107-11
[4]  Barkan A L,Halasz I,Dornfeld K J.Pituitary radiation is ineffective in normalizing plasma insulin-like growth factor Ⅰ in patients with acromegaly[J].Journal of Clinical Endocrinology and Metabolism.1997,82:3187-3191
[5]  Tamatani M,Ogawa S,Tohyama M.Roles of bcl-2 and caspases in hypoxia-induced neuronal cell death:a possible neuroprotective mechanism of peptide growth factors[J].MOLECULAR BRAIN RESEARCH.1998,58:27-39
[6]  Behringer R,Lewin T,Quaife C.Expression of insulin-like growth factor Ⅰ stimulates normal somatic growth in growth hormone-deficient transgenic mice[J].Endocrinology.1990,127(10):33-40
[7]  范卫新,朱文元.肝细胞生长因子对小鼠触须毛囊体外培养的研究[J].中华皮肤科杂志.1999,32(5):11-13
[8]  伍津津,刘荣卿,叶庆佾.人头皮游离毛囊培养[J].中华皮肤科杂志.1996,19(4):8-10
[9]  Itami S,Kurata S,Takayasu S.Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-Ⅰ from dermal papilla cells[J].Biochemical and Biophysical Research Communications.1995,212:988-994
[10]  HyndP.Factors influencing cellular events in the wool follicle[A].New York:Chapman and Hall USA.1989:169-183

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