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禾谷缢管蚜、麦长管蚜乙酰胆碱酯酶基因片段的克隆与序列分析

DOI: 10.7685/j.issn.1000-2030.2006.01.008, PP. 35-39

Keywords: 禾谷缢管蚜,麦长管蚜,乙酰胆碱酯酶,序列分析

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Abstract:

采用RT-PCR技术,设计简并上、下游引物,从禾谷缢管蚜(Rhopalosiphumpadi(Linnaeus))和麦长管蚜(Sito-bionavenae(Fabricius))中均克隆出2种不同乙酰胆碱酯酶的cDNA片段,分别命名为Rp.ace1、Rp.ace2和Sa.ace1、Sa.ace2。同源性分析结果表明,Rp.ace1、Sa.ace1与棉蚜(Aphisgossypii)和桃蚜(Myzuspersicae)的Ⅰ型乙酰胆碱酯酶(Ace1)基因序列具有96%以上的相似性,Rp.ace2和Sa.ace2与棉蚜和桃蚜的Ⅱ型乙酰胆碱酯酶(Ace2)以及从麦二叉蚜(Schizaphisgraminum)中克隆出的乙酰胆碱酯酶基因序列具有95%以上的相似性。说明禾谷缢管蚜和麦长管蚜体内都存在2种乙酰胆碱酯酶基因,而且预示蚜虫体内可能普遍存在2种不同的乙酰胆碱酯酶基因,这2种基因都具有相应的功能。

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