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应用PCR-DGGE指纹图谱技术分析盐水鸭贮藏过程中的细菌多样性

DOI: 10.7685/j.issn.1000-2030.2011.02.024, PP. 135-138

Keywords: 盐水鸭,细菌多样性,16SrDNA,变性梯度凝胶电泳

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Abstract:

利用传统平板培养和变性梯度凝胶电泳(DGGE)指纹图谱相结合的方法,对盐水鸭在4℃贮藏期间的细菌多样性进行了分析。传统培养方法结果显示盐水鸭在贮藏期间细菌数量增加迅速。PCR-DGGE对盐水鸭及平板培养物中的细菌组成分析结果显示腐生葡萄球菌(Staphylococcisaprophyticus)、溶酪大球菌(Macrococcuscaseolyticus)、魏斯氏菌(Weissellasp.)、中度嗜盐菌(Halomonassp.)和盐单胞菌(Cobetiasp.)是盐水鸭的主要细菌,而腐生葡萄球菌、溶酪大球菌和魏斯氏菌是平板培养物中的主要细菌。

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