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转hpalXoo基因棉花活性氧产生及防卫相关基因的表达

DOI: 10.7685/j.issn.1000-2030.2011.02.011, PP. 61-66

Keywords: HarpinXoo,转基因棉花,活性氧,PAL,POD,防卫基因

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Abstract:

采用H2O2和酶活性检测、DAB染色、定量实时PCR等方法研究转hpa1Xoo基因棉花T-34叶组织中的活性氧。结果显示无病原菌侵染时,T-34和出发品种Z35均未产生活性氧。在接种黄萎病菌分生孢子悬浮液后,与Z35相比,在T-34叶组织中接菌0~8hH2O2含量变化呈现2个峰值;接菌8h时PAL和POD酶活性显著增强;DAB染色显示褐红色斑产生;2个防卫基因ghAOX1和hsr203J超表达。这些结果表明,转hpa1Xoo基因棉花为某些防卫基因超水平表达提供了分子基础,使受到病原菌侵染时才诱导产生的高水平活性氧及相关基因表达,并且组成型表达的HarpinXoo赋予转基因棉花对黄萎病菌的抗性。

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