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不同生物有机肥对甜瓜土传枯萎病防治效果及对根际土壤微生物区系的影响

DOI: 10.7685/j.issn.1000-2030.2012.06.010, PP. 55-60

Keywords: 甜瓜枯萎病,生物有机肥,根际微生物,PCR-DGGE,BacillussubtilisY-IV1,TrichodermaharzianumSQR-T37

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Abstract:

采用盆栽试验和平板培养及DGGE技术研究施用不同生物有机肥(BIO)对甜瓜土传枯萎病防治效果和根际土壤微生物区系的影响。结果表明施用生物有机肥使甜瓜生物量和防病率明显增高。在挂果期,含BacillussubtilisY-IV1的BIO1与含TrichodermaharzianumSQR-T37的BIO2混合施用处理的甜瓜鲜质量和干质量分别增加139%、287%,防病率达到了90.9%;施用生物有机肥处理根际细菌和放线菌数量相对于对照明显增多,而真菌和病原菌的数量显著减少。BIO1与BIO2混合施用对根际土壤微生物数量的影响最显著。结论使用混合型生物有机肥对土传病害防治效果最好,明显促进作物生长,改善土壤微生物区系,对连作病害土壤的修复作用显著。

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