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山羊DAZL基因的克隆及在山羊骨髓间充质干细胞中的表达

DOI: 10.7685/j.issn.1000-2030.2012.06.017, PP. 104-110

Keywords: 山羊,DAZL基因,序列分析,真核表达,骨髓间充质干细胞

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Abstract:

本研究以山羊睾丸组织为材料,用RT-PCR法成功克隆了山羊DAZL基因的cDNA序列950bp,测序和生物信息学分析表明,其含有885bp的完整开放阅读框(ORF),编码295个氨基酸,与牛的氨基酸序列同源性为97.97%,编码产物含有典型的RNA识别基序RRM和DAZ重复基序;将山羊DAZL基因亚克隆至表达载体pEGFP-C1上,构建了山羊DAZL基因真核表达载体pEGFP-DAZL,采用脂质体法将其瞬时转染至山羊骨髓间充质干细胞(BMSC)中,通过荧光显微镜观察确定重组质粒在山羊BMSC内的表达和定位。

References

[1]  Xu E Y,Moore F L,Pera R A.A gene family required f or human germ cell development evolved from an ancient meiotic gene conserved in metazoans[J].Proc Natl Acad Sci USA,2001,98(13):7414-7419
[2]  Eberhart C G,Maines J Z,Wasserman S A.Meiotic cell cycle requirement for a fly homologue of human deleted in Azoospermia[J].Nature,1996,381:783-785
[3]  Reijo R A,Dorfman D M,Slee R,et al.DAZ family proteins exist throughout male germ cell development and transit from nucleus to cytoplasm at meiosis in humans and mice[J].Biol Reprod,2000,63(5):1490-1496
[4]  Houston D W,King M L.A critical role for Xdazl,a germ plasmlocalized RNA in the differentiation of primordial germ cells in Xenopus[J].Development,2000,127(3):447-456
[5]  Vogel T,Speed R M,Ross A,et al.Partial rescue of the DAZL knockout mouse by the human DAZL gene[J].Mol Hum Reprod,2002,8(9):797-804
[6]  Nayernia K,Lee J H,Drusenheimer N,et al.Derivation of male germ cells from bone marrow stem cells[J].Lab Invest,2006,86(7):654-663
[7]  Yen P H,ChaiN N,Salido E C.The human autosomal gene DAZLA:testis specificity and a candidate for male infertility[J].Hum Mol Genet,1996,5(12):2013-2017
[8]  Maiwald R,Luche R M,Epstein C J.Isolation of a mouse homolog of the human DAZ(Deleted in Azoospermia)gene[J].Mamm Genome,1996,7(8):628
[9]  李新福,谢元澄,张庆波,等.牦牛DAZL基因编码区的克隆和序列特征与进化分析[J].南京农业大学学报,2010,33(3):109-114
[10]  Porada C D,Zanjani E D,Almeida-Porad G.Adult mesenchymal stem cells:a pluripotent population with multiple applications[J].Curr Stem Cell Res Ther,2006,1(3):365-369
[11]  Rho G J,Kumar B M,Balasubramanian S S.Porcine mesenchymal stem cells-current technological status and future perspective[J].Front Biosci,2009,14:3942-3961
[12]  Ieda M,Fu J D,Delgado-Olguin P,et al.Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors[J].Cell,2010,142(3):375-386
[13]  Vierbuchen T,Ostermeier A,Pang Z P,et al.Direct conversion of fibroblasts to functional neurons by defined factors[J].Nature,2010,463:1035-1041
[14]  Schrans-Stassen B H,Saunders P T,Cooke H J,et al.Nature of the sperma-togenic arrest in Dazl-/-mice[J].Biol Reprod,2001,65:771-776
[15]  张庆波,李齐发,李家璜,等.牛精子发生相关新基因b-DAZL的克隆、生物信息学分析与组织表达研究[J].自然科学进展,2008,18(5):493-504
[16]  Kee K,Angeles V T,Flores M,et al.Human DAZL,DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation[J].Nature,2009,462:222-225
[17]  Yu Z,Ji P,Cao J,et al.Dazl promotes germ cell differentiation from embryonic stem cells[J].J Mol Cell Biol,2009,1(2):93-103
[18]  Haston K M,Tung J Y,Reijo Pera R A.Dazl functions in maintenance of pluripotency and genetic and epigenetic programs of differentiation in mouse primordial germ cells in vivo and in vitro[J].PLoS One,2009,4(5):5654
[19]  Lue Y,Erkkila K,Liu P Y,et al.Fate of bone marrow stem cells transplanted into the testis:potential implication for men with testicular failure[J].Am J Pathol,2007,170(3):899-908
[20]  Drusenheimer N,Wulf G,Nolte J,et al.Putative human male germ cells from bone marrow stem cells[J].Soc Reprod Fertil Suppl,2007,63:69-76
[21]  Houston D W,Zhang J,Maines J Z,et al.A Xenopus DAZ-like gene encodes an RNA component of germ plasm and is a functional homologue of Drosophila boule[J].Development,1998,125(2):171-180
[22]  Yen P H.Putative biological functions of the DAZ family[J].Intl J Andro,2004,27(3):125-129
[23]  Linher K,Cheung Q,Baker P,et al.An epigenetic mechanism regulates germ cell specific expression of the porcine Deleted in Azoospermia-Like(DAZL)gene[J].Differentiation,2009,77(4):335-349

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