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桔小实蝇takeout基因的克隆和序列分析及时空表达

DOI: 10.7685/j.issn.1000-2030.2012.04.014, PP. 75-80

Keywords: 桔小实蝇,Takeout蛋白,基因克隆,荧光定量PCR

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Abstract:

采用RT-PCR及RACE技术克隆了桔小实蝇(Bactroceradorsalis(Hendel))takeout基因的cDNA全长序列,命名为BdorTO。测序结果表明BdorTO开放阅读框全长747bp,编码248个氨基酸,相对分子质量约为28.15×103,等电点为5.29。SignalP软件分析表明,该蛋白N端具21个氨基酸的信号肽,为分泌型蛋白。氨基酸序列比对表明,BdorTO蛋白与其他昆虫TO蛋白的序列一致性较低,其中与刺舌蝇(Glossinamorsitans)的序列一致性最高,一致性值为48.6%。荧光定量PCR分析表明,BdorTO在触角中的表达量最高,推测BdorTO可能参与了桔小实蝇嗅觉感受过程;在雄虫生殖节中的表达量明显高于雌虫生殖节,提示BdorTO可能与桔小实蝇雄虫的生殖生理过程密切相关。

References

[1]  So W V,Sarov-Blat L,Kotarski C K,et al.takeout,a novel Drosophila gene under circadian clock transcriptional regulation[J].Molecular and Cellular Biology,2000,20(18):6935-6944
[2]  Saito K,Su Z H,Emi A,et al.Cloning and expression analysis of takeout/JHBP family genes of silkworm,Bombyx mori[J].Insect Molecular Biology,2006,15(3):245-251
[3]  Justice R W,Dimitratos S,Walter M F,et al.Sexual dimorphic expression of putative antennal carrier protein genes in the malaria vector Anopheles gambiae[J].Insect Molecular Biology,2003,12(6):581-594
[4]  Meunier N,Belgacem Y H,Martin J R.Regulation of feeding behaviour and locomotor activity by takeout in Drosophila[J].The Journal of Experimental Biology,2007,210:1424-1434
[5]  Bauer J,Antosh M,Chang C,et al.Comparative transcriptional profiling identifies takeout as a gene that regulates life span[J].Aging,2010,2(5):298-310
[6]  Benito J,Hoxha V,Lama C,et al.The circadian output gene takeout is regulated by Pdp1ε[J].Proc Natl Acad Sci USA,2010,107:2544-2549
[7]  Fujikawa K,Seno K,Ozaki M.A novel Takeout-like protein expressed in the taste and olfactory organs of the blowfly,Phormia regina[J].The FEBS Journal,2006,273(18):4311-4321
[8]  Sarov-Blat L,So W V,Liu L,et al.The Drosophila takeout gene is a novel molecular link between circadian rhythms and feeding behavior[J].Cell,2000,101(6):647-656
[9]  Dauwalder B,Tsujimoto S,Moss J,et al.The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior[J].Genes & Development,2002,16:2879-2892
[10]  Du J G,Hiruma K,Riddiford L M.A novel gene in the takeout gene family is regulated by hormones and nutrients in Manduca larval epidermis[J].Insect Biochemistry Molecular Biology,2003,33(8):803-814
[11]  Zhu H,Casselman A,Reppert S M.Chasing migration genes:a brain expressed sequence tag resource for summer and migratory monarch butterflies(Danaus plexippus)[J].PLoS ONE,2008,3(1):e1345
[12]  Guo W,Wang X,Ma Z,et al.CSP and takeout genes modulate the switch between attraction and repulsion during behavioral phase change in the migratory locus[J].PLoS Genetics,2011,7(2):e1001291
[13]  Alyokhin A V,Mille C,Messing R H,et al.Selection of pupation habitats by oriental fruit fly larvae in the laboratory[J].Journal of Insect Behavior,2001,14(1):57-68
[14]  Nakahara S,Kobashigawa Y,Muraji M.Genetic variations among and within populations of the oriental fruit fly,Bactrocera dorsalis(Diptera;Tephritidae),detected by PCR-RFLP of the mitochondrial control region[J].Appl Entomol Zool,2008,43(3):457-465
[15]  Hsu J C,Feng H T.Insecticide susceptibility of the oriental fruit fly(Bactrocera dorsalis(Hendel)(Diptera:Tephritidae)in Taiwan[J].Chinese Journal of Entomology,2000,20:109-118
[16]  章玉苹,曾玲,陆永跃,等.华南地区桔小实蝇抗药性动态监测[J].华南农业大学学报,2007,28(3):20-23
[17]  袁盛勇,孔琼,肖春,等.桔小实蝇成虫人工饲料的筛选[J].华中农业大学学报,2006,25(4):371-374
[18]  Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408

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