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水稻白叶枯病菌tatB基因的克隆及功能分析

DOI: 10.7685/j.issn.1000-2030.2012.03.011, PP. 57-63

Keywords: 水稻白叶枯病菌,tatB基因,致病性,胞外多糖

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Abstract:

根据水稻白叶枯病菌(Xanthomonasoryzaepv.oryzae,Xoo)PXO99的tatB基因序列设计引物,采用PCR方法从PXO99中克隆了tatB基因,命名为tatBXoo。通过同源重组的方法,构建了tatBXoo的插入突变株TBM,在含有X-gluc的抗性平板上筛选,并经Southernblot杂交验证确认。表型测定结果表明与野生型相比,tatB突变株在水稻(寄主)上的致病性减弱,但在烟草(非寄主)上仍具有引起过敏反应的能力;tatB突变导致Xoo致病相关的重要表型如胞外多糖减少,游动性及趋化性减弱。但是,tatB突变不影响Xoo在基本培养基(MMX)中正常生长、胞外纤维素酶产生和生物膜形成。

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