全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

培矮64S/93-11重组自交系分子图谱构建及千粒重QTL检测

DOI: 10.7685/j.issn.1000-2030.2011.01.002, PP. 8-13

Keywords: 水稻,重组自交系,SSR,分子标记,遗传连锁图谱,千粒重,染色体片段置换系,QTL

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用光、温敏核不育系培矮64S与常规品种93-11进行杂交,以单粒传方法建立含217个单株的重组自交系(RILs)群体。选用775对SSR引物进行亲本多态性筛选,共有170对检测到多态性,频率为21.9%。构建的水稻分子遗传图谱共包含141个标记座位,总图距约2060.4cM,标记间平均图距为14.6cM。群体中标记偏分离情况较严重。以该RILs群体217个株系为材料,对千粒重性状进行了QTL分析。结果表明在RILs群体中检测到3个与千粒重相关的QTL,分别在第1、5、8染色体上,命名为qTGW-1、qTGW-5、qTGW-8,其LOD值为5.51、3.31、4.94,贡献率为17.90%、6.12%、9.59%。qTGW-1控制千粒重的增效基因来自低值亲本培矮64S,qTGW-5和qTGW-8控制千粒重的增效基因来自高值亲本93-11。通过相应的含有154个家系的全基因组染色体片段置换系(CSSLs)群体图示基因型分析,证实了在第1染色体上标记RM315附近存在控制千粒重的增效基因,来源于染色体片段供体亲本培矮64S,使千粒重增加1.03g;在第5、8染色体上标记RM3663、RM310附近存在控制千粒重的增效基因,来自染色体片段受体亲本93-11,使千粒重分别增加0.63g和0.80g。

References

[1]  邹江石,王才林. 两系杂交稻"两优培九"的选育及其栽培特性[J]. 中国农业科学, 2003
[2]  Yu,J,Wang,J,Wang,J. A dr’. fft sequence of the rice genome(Oryza sativa L. ssp. indica][J]. Science, 2002
[3]  Xiao,Y,H,Jiang,L,Jiang,L. Development of the chromosome segment substitution lines(CSSLs]derived from a hybrid rice cross,Peiai64S/93-11 with super high yield potential[J]. Rice Genetics Newsletter, 2005
[4]  Dellaporta,S,L,Hicks,J,B,Hicks,J,B. A plant DNA minipreparation:version I1[J]. Plant Molecular Biology Reporter, 1983
[5]  Chen,X,Xu,Y,Xu,Y. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L. ][J]. Theoretical and Applied Genetics, 1997
[6]  Wang,S,C,Zeng,Z,B,Zeng,Z,B. Windows QTL Cartographer 2. 5 User Manual[EB/OL]blip://statgen. ncsu. edu/-shchwang/WinQTLCart,pdf, 2009-11-19
[7]  Churchill,G,A. Empirical threshohl values for quantitative trait mapping[J]. Genetika, 1994
[8]  McCouch,S,R,Yano,M,Yano,M. Report on QTL nomenclature[J]. Rice Genetics Newsletter, 1997
[9]  Jiang,G,H,Li,X,H,Li,X,H. Characterization of the genetic basis for yield and its component traits of rice revealed by doubled haphfid population[J]. 遗传学报, 2004
[10]  Thomson,M,J,McClung,A,M,McClung,A,M. Mapping quantitative trait loci for yield,yield components and morpholological traits in an advancedhackcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson[J]. Theoretical and Applied Genetics, 2003
[11]  鄂志国,庄杰云. 水稻千粒重QTL图谱整合研究[J]. 科技通报, 2008
[12]  曹立勇,庄杰云. 水稻产量性状的QTL定位与上位性分析[J]. 中国农业科学, 2003
[13]  Hittalmani,S,Bagali,P,G,Bagali,P,G. Molecular mapping of quantitative trait loci for plant growth,yield and yield related traits across three diverse locations in a doublcd haploid rice population[J]. Euphytica, 2002
[14]  Yoon,D,B,Kim,H,J,Kim,H,J. Mapping quantitative trait loci for yield components and morphological traits in an advanced backeross population between Oryza grandiglumis and the O. sativa japonica cultivar Hwascongbyeo[J]. Theoretical and Applied Genetics, 2006
[15]  Song,X,J,Shi,M,Shi,M. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase[J]. Nature Genetics, 2007
[16]  Wang,W,W,Ge,X,M,Ge,X,M. Protcomic profiling of rice embryos from a hybrid rice cultivar and its parental lines[J]. Proteomics, 2008
[17]  Moneada,P,Borrero,J,Borrero,J. Quantitative trait loci for yieht aml yield components in an Oryza sativaxOryza rufipogon BC2F2population evaluated in an upland envirnnment[J]. Theoretical and Applied Genetics, 2001
[18]  Septiningsih,E,M,Lubis,E,Lubis,E. Identification of quantitative trait loci for yield and yield components in an advanced backerosspopulation derived from the Oryza sativa variety IR64 and the wiht relative O. rufipogon[J]. Theoretical and Applied Genetics, 2003
[19]  Lu,C,F,Tan,Z,B,Tan,Z,B. Comparative mapping of QTLs for agronomic traits of rice across environments by using a doubled-haploid pop-ulation[J]. Theoretical and Applied Genetics, 1997
[20]  Lin,H,X,Zhuang,J,Y,Zhuang,J,Y. RFLP mapping of QTLs for yield and related characters in rice(Oryza sativa L. ][J]. Theoretical and Applied Genetics, 1996
[21]  Temnykh,S,Ayres,N,Ayres,N. Mapping and genome organization of microsatellite sequences in rice(Oryza sativa L. )[J]. Theoretical and Applied Genetics, 2000
[22]  Tan,L,B,Liu,F,X,Liu,F,X. Quantitative trail loci underlying domestication and yield-related traits in an Qryza sativaxQryza rufipogon advanced backcross population[J]. Genome/National Research Council Canada=genome/Conseil National De Recherches Canada, 2008

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133