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运输应激对猪淋巴结IL-2、IL-6和IL-10及其受体mRNA转录的影响

DOI: 10.7685/j.issn.1000-2030.2011.01.018, PP. 95-100

Keywords: ,运输应激,肠系膜淋巴结,细胞因子,P38MAPK,核因子κB

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Abstract:

为研究运输应激对猪肠系膜淋巴结中IL-2、IL-6、IL-10及其受体mRNA转录的影响,并探讨p38丝裂原活化蛋白激酶(P38MAPK)和核因子κB(NF-κB)对其转录的调控作用,用荧光定量RT-PCR方法对肠系膜淋巴结中3种细胞因子及其受体mRNA进行定量检测,用ELISA方法检测猪肠系膜淋巴结组织匀浆液中P38MAPK含量的动态变化,用激光扫描共聚焦显微镜观察运输前后NF-κB在肠系膜淋巴结细胞中的分布。结果显示经1、2和4h的运输应激,肠系膜淋巴结中3种细胞因子的转录均发生了显著变化,且均是4h时达到最大值,并极显著高于对照组、1h组和2h组(P<0.01),而其相应受体的变化趋势不同。NF-κB在肠系膜淋巴结细胞中的表达呈逐渐上升趋势,到4h时达到最大,其入核率也是同样的变化趋势。运输应激过程中P38MAPK含量没有明显的变化。结论运输应激可引起猪肠系膜淋巴结细胞因子及其受体mRNA转录的急剧变化,且存在时效性;NF-κB的激活是调控猪肠系膜淋巴结相关细胞因子变化的一个重要因素。

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