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不同氟烷基因型猪脂肪组织中perilipin基因及糖皮质激素作用相关基因的表达

DOI: 10.7685/j.issn.1000-2030.2011.01.016, PP. 84-88

Keywords: ,perilipin基因,背脂,肾周脂,氟烷基因

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Abstract:

为研究脂滴包被蛋白(Perilipin)在猪脂肪组织中的表达分布情况及其与糖皮质激素作用的关系,选取氟烷基因隐性纯合子猪(Halnn)和氟烷基因显性纯合子猪(HalNN)为动物模型,采用半定量实时荧光PCR(rqRT-PCR)技术分析其背脂和肾周脂中perilipin基因及脂肪代谢和糖皮质激素作用相关基因的表达。结果显示两种氟烷基因型猪的体重、背膘厚和背膘指数无显著差异,但Halnn肾周脂重(P=0.09)和肾周脂指数(P=0.10)有低于HalNN猪的趋势。双因子方差分析结果表明Halnn猪脂肪组织中perilipinmRNA水平显著高于HalNN猪(P<0.05),而糖皮质激素受体基因(GR)表达水平显著低于HalNN猪(P<0.05),11β-羟基类固醇脱氢酶1基因(11β-HSD1)、脂肪酸合成酶基因(FAS)和激素敏感酯酶基因(HSL)表达水平在两种基因型之间无显著差异。背脂中perilipin和GR的表达水平与肾周脂中的无显著差异,背脂中11β-HSD1和FAS表达水平显著高于肾周脂(P<0.05),而HSL在背脂中的表达情况正好相反(P<0.05)。以上结果提示HalNN与Halnn猪脂肪组织中perilipin基因表达存在显著差异,且这种差异可能与GR有关;背脂和肾周脂的脂肪代谢模式可能存在差异。

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