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黄瓜染色体片段导入系的构建与遗传评价

DOI: 10.7685/j.issn.1000-2030.2011.01.004, PP. 20-24

Keywords: 黄瓜,SSR标记,染色体片段导入系,评价

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Abstract:

以综合性状优良的栽培黄瓜(CucumissativusL.,2n=14)北京截头为受体亲本,以携带有多种抗性性状的黄瓜珍稀野生种酸黄瓜(CucumishystrixChakr.,2n=24)为供体亲本,在本实验室前期获得种间杂交异源四倍体的基础上,通过多代回交和自交,结合SSR标记辅助选择构建了以北京截头为遗传背景的黄瓜野生种染色体片段导入系,并对其导入片段的数目、分布、大小和覆盖率等进行了评价。结果表明该导入系群体携带的38个供体片段不均匀地分布于黄瓜7条染色体上,其长度介于1.1~14.9cM,平均长度为5.3cM,总长度为201.9cM,在黄瓜基因组上的覆盖率为23.5%。

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