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IGF-1促进山羊瘤胃上皮细胞增殖的机制

DOI: 10.7685/j.issn.1000-2030.2014.05.017, PP. 106-110

Keywords: IGF-1,IGF-1R,胞外调节蛋白激酶(ERK),cyclinD1,细胞增殖,瘤胃上皮

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Abstract:

通过体外试验研究胰岛素样生长因子1(IGF-1)对山羊瘤胃上皮细胞增殖的影响,胰岛素样生长因子1型受体(IGF-1R)以及受体后信号转导途径在此过程中所起的作用。试验采用IGF-1、IGF-1R抑制剂分别处理体外培养的瘤胃上皮细胞,研究IGF-1对瘤胃上皮细胞增殖、细胞周期蛋白表达及增殖相关信号转导途径中关键蛋白的活化水平;并用添加胞外调节蛋白激酶(ERK)抑制剂、蛋白激酶-B(AKT)抑制剂处理,研究增殖相关信号转导途径在IGF-1诱导瘤胃上皮细胞增殖中的作用。结果表明IGF-1显著提高体外培养瘤胃上皮细胞3H-TdR掺入率(P0.05);而IGF-1R抑制剂(P<0.05)、ERK抑制剂(P<0.05)处理均能反转IGF-1对瘤胃上皮细胞cyclinD1蛋白的促表达作用。结论IGF-1与IGF-1R结合后,可以激活下游Ras/Raf/MEK/ERK信号转导途径,提高cyclinD1表达,促进瘤胃上皮细胞增殖。

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