[目的]大豆疫霉根腐病是大豆毁灭性的病害之一,防治该病最安全有效的方法是培育和利用抗性品种。迄今为止,已在大豆基因组上鉴定了21个大豆疫霉根腐病抗性基因,但是在中国只有少数基因(如Rps1c、Rps1k等)抗性有效。因此,急需发掘新的大豆疫霉根腐病抗性基因,以满足抗病育种的需要。‘大方六月早’对大豆疫霉菌的抗谱较广,是目前筛选出的优异抗源。[方法]采用下胚轴创伤接种方法进行抗病性鉴定,以品种‘大方六月早’为抗病亲本与品种‘矮脚早’配置杂交组合获得F2:3代家系群体,并进行遗传分析,通过SSR(simple sequence repeat)标记对‘大方六月早’的抗性基因进行定位。[结果]该群体的抗性遗传分析表明:‘大方六月早’对大豆疫霉根腐病的抗性由1对主效基因控制,抗病对感病表现为显性,暂命名为RpsAH。使用SSR标记进行分子作图,抗病基因RpsAH被定位在大豆3号染色体(即N连锁群)上,距离标记Sat_166为4.1 cM。[结论]大豆品种‘大方六月早’对大豆疫霉菌株AH的抗性由1个单显性基因控制,该基因定位于大豆分子遗传图谱N连锁群上。[Objectives]Phytophthora root and stem rot(PRR),caused by Phytophthora sojae is a destructive disease on soybean throughout the world. Utilization of resistance varieties is the most economical and environmentally safe method for controlling the disease. Up to now,21 resistance genes have been identified in soybean. However,only a few genes,such as Rps1c and Rps1k,were effectively resistant to populations of P.sojae in China,so to mine new resistance genes is necessary for the disease. Soybean cultivar‘Dafangliuyuezao’has broad spectrum resistance to P.sojae,and is an elite resistance source for Phytophthora root rot of soybean. [Methods]A population of F2:3 lines derived from the cross of‘Dafangliuyuezao’בAijiaozao’was developed for resistance genetic analysis and resistance gene mapping. Using hypocotyl inoculation method at the seedling stage in the glasshouse,the reaction to P.sojae isolate AH(virulence type 2,3a,3b,4,5),in 156 F2:3 lines were identified. [Results]The segregation ratio in the population fit into 1:2:1 for homozygous resistant,segregating and homozygous susceptible,showing that the resistance of ‘Dafangliuyuezao’to P.sojae strain AH is controlled by a single dominant gene,with the temporary name of RpsAH. On the basis of linkage analysis with SSR markers,RpsAH was located on molecular linkage group N,and flanked by Sat_166 with genetic distances 4.1 cM. [Conclusions]The resistance to Phytophthora root rot is controlled by a single dominant gene,and the resistance gene RpsAH in‘Dafangliuyuezao’was mapped on molecular linkage group N
References
[1]
Schmitthenner A F. Problems and progress in control Phytophthora root rot of soybean[J]. Plant Disease,1985,69:362-368
[2]
许修宏,吕慧颖,曲娟娟,等. 大豆疫霉根腐病菌分离方法及发病地区初步调查[J]. 大豆科学,2002,21(2):147-150 [Xu X H,Lü H Y,Qu J J,et al. Isolation method for Phytophthora sojae and investigation for the infected regions[J]. Soybean Science,2002,21(2):147-150(in Chinese with English abstract)]
[3]
马淑梅,丁俊杰,郑天琪,等. 黑龙江省大豆疫霉根腐病生理小种鉴定结果[J]. 大豆科学,2005,24(4):260-262 [Ma S M,Ding J J,Zheng T Q,et al. The identification of physiological races of Phytophthora megasperma[J]. Soybean Science,2005,24(4):260-262(in Chinese with English abstract)]
[4]
王焕如,朱之？. 选育和利用抗病品种策略问题的讨论[J]. 植物保护,1992,18(2):28-30 [Wang H R,Zhu Z Y. Strategy for breeding and utilization of resistant varieties[J]. Plant Protection,1992,18(2):28-30(in Chinese)]
[5]
孙石,赵晋铭,武晓玲,等. 大豆对大豆疫霉根腐病抗性的遗传分析[J]. 中国农业科学,2009,42(2):492-498 [Sun S,Zhao J M,Wu X L,et al. Inheritance of resistance to Phytophthora sojae in soybean[J]. Scientia Agricultura Sinica,2009,42(2):492-498(in Chinese with English abstract)]
[6]
Buzzell R I,Anderson T R. Inheritance and race reaction of a new soybean Rps1 allele[J]. Plant Disease,1992,76:600-601
[7]
Gao H Y,Bhattacharyya M K. The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences[J]. BMC Plant Biology,2008,8:29
[8]
Rennie B D,Buzzell R I,Anderson T R,et al. Evaluation of four Japanese soybean cultivars for Rps alleles conferring resistance to Phytophthora megasperma f.sp.glycine[J]. Canadian Journal of Plant Science,1992,72:217-220
[9]
Cregan P B,Jarvik T,Bush A L,et al. An integrated genetic linkage map of soybean genome[J]. Crop Science,1999,39:1464-1490
[10]
Demirbas A,Rector B G,Lohnes D G,et al. Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance[J]. Crop Science,2001,41:1220-1227
[11]
Sugimoto T,Yoshida S,Watanabe K,et al. Identification of SSR markers linked to the Phytophthora resistance gene Rps1-d in soybean[J]. Plant Breeding,2008,127:154-159
[12]
Weng C,Yu K,Anderson T R,et al. Mapping genes conferring resistance to Phytophthora root rot of soybean,Rps1a and Rps7[J]. The Journal of Heredity,2001,92:442-446
[13]
Sandhu D,Schallock K G,Rivera-Velez N,et al. Soybean Phytophthora resistance gene Rps8 maps closely to the Rps3 region[J]. The Journal of Heredity,2005,96:536-541
[14]
Gordon S G,Martin S K,Dorrance A E. Rps8 maps to a resistance gene rich region on soybean molecular linkage group F[J]. Crop Science,2006,46:168-173
[15]
Lin F,Zhao M X,Ping J Q,et al. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B[J]. Theoretical and Applied Genetics,2013,126:2177-2185
[16]
Sun S,Wu X L,Zhao J M,et al. Characterization and mapping of RpsYu25,a novel resistance gene to Phytophthora sojae[J]. Plant Breeding,2011,130:139-143
[17]
Sugimoto T,Yoshida S,Kaga A,et al. Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge[J]. Euphytica,2011,182:133-145
[18]
Wu X L,Zhang B Q,Sun S,et al. Identification,genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean[J]. Agricultural Sciences in China,2011,10:1506-1511
[19]
Zhang J Q,Xia C J,Wang X M,et al. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar[J]. Theoretical and Applied Genetics,2013,126:1555-1561
[20]
范爱颖,王晓鸣,方小平,等. 大豆品种豫豆25抗疫霉根腐病基因的鉴定[J]. 作物学报,2009,35(10):1844-1850 [Fan A Y,Wang X M,Fang X P,et al. Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou 25[J]. Acta Agronomica Sinica,2009,35(10):1844-1850(in Chinese with English abstract)]
[21]
Sun J T,Li L H,Zhao J M,et al. Genetic analysis and fine mapping of RpsJS,a novel resistance gene to Phytophthora sojae in soybean[Glycine max(L.)Merr.][J]. Theoretial and Applied Genetics,2014,127:913-919
[22]
陈晓玲,朱振东,王晓鸣,等. 大豆品种(系)抗疫霉根腐病基因推导[J]. 中国农业科学,2008,41(4):1227-1234 [Chen X L,Zhu Z D,Wang X M,et al. Postulation of Phytophthora resistance genes in soybean cultivars or lines[J]. Scientia Agricultura Sinica,2008,41(4):1227-1234(in Chinese with English abstract)]
[23]
徐鹏飞,吴俊江,范素杰,等. 大豆疫霉根腐病菌的分离鉴定及种质资源对3号生理小种的抗性评价[J]. 大豆科学,2010,29(2):272-275 [Xu P F,Wu J J,Fan S J,et al. Isolation of Phytophthora sojae and idendification of resistance to race 3 in soybean germplasm[J]. Soybean Science,2010,29(2):272-275(in Chinese with English abstract)]
[24]
Song Q J,Marek L F,Shoemaker R C,et al. A new integrated genetic linkage map of the soybean[J]. Theoretial and Applied Genetics,2004,109:122-128
[25]
Collard B C Y,Mackill D J. Marker-assisted selection:an approach for precision plant breeding in the twenty-first century[J]. Philosophical Transactions of the Royal Society B:Biological Sciences,2008,363:557-572
[26]
Wrather J A,Koenning S R. Effects of diseases on soybean yields in the United States 1996 to 2007[J]. Plant Health Progress,2009. doi:10.1094/PHP-2009-0401-01-RS
[27]
沈崇尧,苏彦纯. 中国大豆疫霉病菌的发现及初步研究[J]. 植物病理学报,1991,21(4):298 [Shen C Y,Su Y C. Discovery and preliminary studies of Phytophthora megasperma on soybean in China[J]. Acta Phytopathologica Sinica,1991,21(4):298(in Chinese)]
