[目的]探索乳酸作为强化生物除磷(EBPR)进水碳源的可行性.[方法]首先采用厌氧-好氧型序批式生物反应器,以乳酸为唯一进水碳源驯化EBPR污泥(SBR-2),并与以乙酸为唯一进水碳源的EBPR污泥(SBR-1)对比;待SBR-2达到稳态运行后,在1个运行周期定期取样,测定混合液中化学需氧量(COD)、磷酸根、氨氮和硝氮等参数的周期变化,并与SBR-1比较;通过研究不同进水磷浓度对2个反应器除磷效率的影响,比较2种EBPR污泥的除磷能力.[结果]以乙酸为进水碳源的SBR-1和以乳酸为进水碳源的SBR-2分别经过8和31 d的驯化期实现了稳定的EBPR功能;与乙酸驯化的EBPR污泥类似,乳酸驯化所得的EBPR污泥也表现出典型聚磷菌(PAO)的代谢特征.以乳酸为碳源驯化而成的EBPR污泥其主要特征参数如有机酸吸收速率、磷释放速率和磷吸收速率均明显低于以乙酸为碳源驯化而成的EBPR污泥,但是由于其厌氧释放的磷浓度较低,结果仍可在相同的时间内完成磷的吸收.在进水磷酸根质量浓度小于等于12 mg？L-1时,以乳酸为进水碳源的反应器可以实现稳定高效的除磷效果,出水磷酸根质量浓度稳定在0.3 mg？L-1以下.[结论]采用乳酸作为EBPR的进水碳源是可行的.[Objectives]Objective of the present study was to explore the feasibility of enhanced biological phosphorus removal(EBPR)by using lactic acid as the sole carbon source. [Methods]Acclimation of EBPR sludge was carried out in an anaerobic-aerobic sequencing batch reactor(SBR)by using lactic acid as influent carbon source(SBR-2)and compared with the control SBR, which received acetic acid as influent carbon source(SBR-1). Once steady state operation was achieved in SBR-2, a cyclic study for both reactors were initiated through periodic sampling and analysis of such parameters as chemical oxygen demand(COD), phosphate, ammonia and nitrate in the mixed liquor. EBPR sludge’s ability in phosphorus removal was compared between two SBRs through investigating the effects of influent P concentration on phosphate removal efficiency. [Results]In the present study, enhanced biological phosphorus removal was successfully established in both lactic acid-feed SBR-2 and acetic acid-feed SBR-1 after an acclimation period of 8 d and 31 d, respectively. Similar to acetic acid-feed EBPR sludge, lactic acid-feed EBPR sludge exhibited characteristic metabolic behavior typical to polyphosphate-accumulating organisms(PAOs). As compared to acetic acid-feed EBPR sludge, lactic acid-feed EBPR sludge was characterized by significantly lower values of such parameters as organic acid uptake rate, P-release rate and P-uptake rate. However this did not lead to any difference in the time period required for complete aerobic P-uptake between these two sludges and this could be attributed to significantly lower P-release observed in the lactic acid-feed SBR-2. When influent phosphate concentration was not more than 12 mg？L-1, nearly complete phosphorus removal could be achieved for the lactic acid-feed SBR-2 as indicated by low levels of effluent phosphate concentration below 0.3 mg？L-1. [Conclusion]EBPR could be achieved by using lactic acid as the sole carbon source
References
[1]
Zhang B,He P J,Ye N F,et al. Enhanced isomer purity of lactic acid from the non-sterile fermentation of kitchen wastes[J]. Bioresource Technology,2008,99:855-862
[2]
王旭明,汪群慧,任南琪,等. 解淀粉乳酸细菌在厨余垃圾乳酸发酵中的应用[J]. 环境科学,2006,27(4):800-803 [Wang X M,Wang Q H,Ren N Q,et al. Isolation of an amylolytic lactic acid bacterium and its application on lactic acid production from kitchen waste[J]. Environmental Science,2006,27(4):800-803(in Chinese with English abstract)]
[3]
国家环境保护总局. 水和废水检测分析方法[M]. 4版. 北京:中国环境科学出版社,2002 [State Environmental Protection Administration. Analytical Methods for the Monitoring of Water and Wastewater[M]. 4th ed. Beijing:China Environmental Science Press,2002(in Chinese)]
[4]
李夕耀,彭永臻,王淑莹,等. 聚磷菌胞内多聚物的分析检测方法[J]. 四川环境,2009,28(2):106-111 [Li X Y,Peng Y Z,Wang S Y,et al. Analyticalmethod for intracellular polymers of polyphosphate accumulating organisms[J]. Sichuan Environment,2009,28(2):106-111(in Chinese with English abstract)]
[5]
Wentzel M C,Ekama G A,Loewenthal R E,et al. Enhanced polyphosphate organism cultures in activated sludge. Part Ⅱ:Experimental behaviour[J]. Water SA,1989,15(2):71-88
[6]
Smolders G J F,van der Meij J,van Loosdrecht M C M. A structured metabolic model for anaerobic and aerobic stoichiometry and kinetics of the biological phosphorus removal processes[J]. Biotechnology and Bioengineering,1995,47:277-287
[7]
张玲玲,陈立,郭兴芳,等. 南北方污水处理厂进水水质特性分析[J]. 给水排水,2012,38(1):45-49 [Zhang L L,Chen L,Guo X F,et al. Analysis of influent quality of municipal wastewater treatment plants in southern and northern areas in China[J]. Water and Wastewater Engineering,2012,38(1):45-49(in Chinese with English abstract)]
[8]
孙迎雪,吴光学,胡洪营,等. 昆明市污水处理厂进水水质特征分析[J]. 环境科学与技术,2013,36(7):147-152 [Sun Y X,Wu G X,Hu H Y,et al. Characteristics of influent quality of municipal wastewater treatment plants in Kunming city[J]. Environmental Science and Technology,2013,36(7):147-152(in Chinese with English abstract)]
[9]
Zou S Q,Yao S,Ni J R. High-efficient nitrogen removal by coupling enriched autotrophic-nitrification and aerobic-denitrification consortiums at cold temperature[J]. Bioresource Technology,2014,161:288-296
[10]
Shoda M,Ishikawa Y. Heterotrophic nitrification and aerobic denitrification of high-strength ammonium in anaerobically digested sludge by Alcaligenes faecalis strain No.4[J]. Journal of Bioscience and Bioengineering,2014,117(6):737-741
[11]
van Loosdrecht M C M,Hooijmans C M,Brdjanovic D,et al. Biological phosphate removal processes[J]. Appl Microbiol Biotechnol,1997,48:289-296
[12]
Blackall L L,Crocetti G R,Saunders A,et al. A review and update of the microbiology of enhanced biological phosphorus removal in wastewater treatment plants[J]. Antonie van Leeuwenhoak,2002,81:681-691
[13]
Taya C,Guerrero J,Vanneste G,et al. Methanol-driven enhanced biological phosphorus removal with a syntrophic consortium[J]. Biotechnology and Bioengineering,2013,110(2):391-400
[14]
Puig S,Coma M,Monclus H,et al. Selection between alcohols and volatile fatty acids as external carbon sources for EBPR[J]. Water Research,2008,42:557-566
[15]
Guerrero J,Taya C,Guisasola A,et al. Glycerol as a sole carbon source for enhanced biological phosphorus removal[J]. Water Research,2012,46:2983-2991
[16]
Begum S A,Batista J R. Microbial selection on enhanced biological phosphorus removal systems fed exclusively with glucose[J]. World J Microbiol Biotechnol,2012,28:2181-2193
[17]
Tong J,Chen Y G. Recovery of nitrogen and phosphorus from alkaline fermentation liquid of waste activated sludge and application of the fermentation liquid to promote biological municipal wastewater treatment[J]. Water Research,2009,43:2969-2976
[18]
张波,何品晶,邵立明. pH和发酵时间对厨余垃圾发酵产乳酸及光学特性的影响[J]. 环境科学,2007,28(4):881-885 [Zhang B,He P J,Shao L M. Effects of pH and fermentation time on yield and optical purity of lactic acid from kitchen wastes fermentation[J]. Environmental Science,2007,28(4):881-885(in Chinese with English abstract)]
