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应用缺口末端标记法(TUNEL)分析犬瘟热病毒(CDV)诱导的细胞凋亡

DOI: 10.7685/j.issn.1000-2030.2000.01.018, PP. 71-73

Keywords: 犬瘟热病毒,细胞凋亡,缺口末端标记法

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Abstract:

利用犬瘟热病毒(caninedistempervirus,CDV)弱毒标准株OPCDV感染敏感细胞系非洲绿猴肾细胞(Vero),应用缺口末端标记法(TdTmediateddUTPnickendlabeling,TUNEL)分析感染细胞的凋亡。结果在感染后48h的Vero细胞中检出了凋亡阳性细胞,而对照细胞内未检测到凋亡细胞。感染后24h的细胞尚未出现细胞病变,这一时期的细胞内也未检测到凋亡细胞。表明CDV能诱导细胞调亡,且凋亡细胞的检出时间与细胞病变的出现时间呈正向相关。

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