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水稻杂种一代及其亲本分蘖期根系基因的差异表达

DOI: 10.7685/j.issn.1000-2030.2000.01.001, PP. 1-4

Keywords: 水稻,根系,分蘖期,mRNA差异显示,基因表达

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Abstract:

以汕优63组合为材料,运用mRNA差异显示技术研究了亲本与杂种一代分蘖期根系基因的差异表达。结果表明,与苗期相比,分蘖期根系基因的表达在质和量上都有显著的改变。质的改变有F1特异表达的基因,F1减弱表达的基因,F1表现沉默(含父强母弱和父弱母强)和F1增强表达;量的改变有F1弱势表达,F1强势表达2种类型。目前已回收了部分差异的cDNA条带,其中差异条带RT1的Northern杂交证明,它在杂种一代弱势表达,在亲本中强势表达。

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