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利用回交重组自交群体检测水稻耐低温发芽数量性状基因座

DOI: 10.7685/j.issn.1000-2030.2007.01.001, PP. 1-6

Keywords: 水稻,低温发芽率,贮藏条件,QTL定位

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Abstract:

利用Nipponbare/Kasalath//Nipponbare回交重组自交系群体,对贮藏在低温(4℃)和室温条件下的种子进行低温发芽率的观察和统计分析,利用WindowsQTLCartographer1.16软件,对控制水稻种子低温发芽力的QTL进行检测。结果发现,低温发芽力QTL分布于第1、5、6、8、9和12染色体上,其中位于第5染色体上的qLTG-5(C597-R3166)和第9染色体上的qLTG-9(R79-C385)在低温萌发过程中持续表达。qLTG-5只在常温贮藏条件下稳定表达,表明此位点可能受种子贮藏条件的影响;qLTG-9在两种贮藏条件下均稳定表达,表明该位点不受种子贮藏条件的影响,可能与种子的耐贮性有关。研究结果可为低温发芽力强的水稻品种的标记辅助选择和图位克隆奠定基础。

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