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北方园艺  2013 

大片段DNA遗传转化的研究进展

, PP. 208-211

Keywords: 克隆载体,大片段,遗传转化?

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Abstract:

DNA克隆技术是分子生物学研究中一项重要的技术手段。随着分子生物学技术的发展及植物基因组计划的进行,产生了既能够克隆大片段DNA又能够将候选基因利用农杆菌介导法进行遗传转化试验的载体。现对大片段克隆载体的发展及大片段DNA遗传转化的影响因素进行综述,并对大片段DNA遗传转化的发展作了展望。????

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