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北方园艺  2013 

滇杨侧芽休眠期基因表达多样性的cDNA-AFLP分析

, PP. 117-121

Keywords: 滇杨侧芽,休眠期,cDNA-AFLP,遗传差异?

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Abstract:

为探讨滇杨分枝特性差异的分子机理,对采集于四川省和云南省的59个滇杨优树无性系3a生苗木进行了cDNA?AFLP差异研究,分析了苗木侧芽休眠时基因表达的多样性。结果表明筛选出的7对引物组合共扩增出了总条带608条,其中有353条差异性条带,Nei’s基因多样性指数为0.1061,Shannon信息指数为0.1856,遗传分化系数为0.0123,居群间的基因流为40.28,表明居群间的遗传分化程度较低。分子生物学方差分析(AMOVA)结果表明,居群间的差异仅为0.99%,差异不显著(P=0.052),居群内差异占99.01%,差异极显著;表明滇杨侧芽在休眠期个体之间基因表达的复杂性;聚类分析结果显示,在亚分枝单元或最小分枝单元,大部分无性系能够以分枝性状相聚。该研究结果为进一步开展滇杨分枝相关基因的筛选及克隆等工作奠定了前期研究基础。?

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