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北方园艺  2013 

农杆菌介导的人白细胞介素-4基因转化甘蓝的研究

, PP. 113-116

Keywords: 甘蓝,白细胞介素4(IL-4),遗传转化?

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Abstract:

以结球甘蓝‘92-1012-3-11’自交系种子转白细胞介素4(IL-4)基因T-0代材料为试材,研究了通过农杆菌介导法将人IL-4基因导入甘蓝后代的遗传转化情况。结表明共得到抗草铵磷(PPT)抗性再生植株396株,聚合酶链式反应(PCR)阳性植株137株,其中带柄子叶转化率为8.1%,下胚轴分化率为5?8%。通过对转基因阳性株T?0代的PPT抗性筛选、PCR、PCR?Southern杂交检测获得IL-4阳性甘蓝植株,表明IL-4基因已转入甘蓝受体中。经ELISA和Western检测,说明IL-4基因已整合到甘蓝基因组,但表达量很低(0.23~2?81ng/mL),且各植株间的表达量差异极大。?

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