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北方园艺  2013 

尖孢镰孢菌番茄专化型的鉴定研究

, PP. 100-103

Keywords: 尖孢镰孢菌,番茄枯萎病,rDNA-ITS,交叉接种?

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Abstract:

以27株镰孢菌菌株为试材,采用菌株形态学观察、rDNA-ITS序列分析以及寄主专化型鉴定相结合的方法,鉴定了尖孢镰孢菌番茄专化型。结果表明经形态学和rDNA-ITS测序筛选,最后筛选出10株尖孢镰孢菌。将尖孢镰孢菌交叉接种到不同寄主植物,确定菌株编号YH070001和DL070009的镰孢菌为尖孢镰孢菌番茄专化型。与单一的传统方法相比,ITSrDNA方法有着较高的灵敏度,所以采取形态学、现代分子生物学以及传统交叉接种相结合的鉴定方式,能够使菌株专化型的鉴定更加准确、完善。?

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